The Carboxyl Terminus of GLUT4 Contains a Serine-Leucine-Leucine Sequence That Functions as a Potent Internalization Motif in Chinese Hamster Ovary Cells
| Autor: | Timothy E. McGraw, Juliet C. Park, Ralph J. Garippa, Rebecca L. Petrush, Amy O. Johnson |
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| Rok vydání: | 1996 |
| Předmět: |
Monosaccharide Transport Proteins
Iron Recombinant Fusion Proteins media_common.quotation_subject Molecular Sequence Data education Mutant Muscle Proteins CHO Cells Biology Biochemistry Serine Chimera (genetics) Leucine Cricetinae Receptors Transferrin Animals Humans Amino Acid Sequence Fluorescent Antibody Technique Indirect Internalization Molecular Biology Peptide sequence DNA Primers media_common chemistry.chemical_classification Alanine Glucose Transporter Type 4 Base Sequence Chinese hamster ovary cell Transferrin Cell Biology Molecular biology Endocytosis Cell Compartmentation Amino acid chemistry |
| Zdroj: | Journal of Biological Chemistry. 271:20660-20668 |
| ISSN: | 0021-9258 |
| Popis: | To characterize the trafficking motifs contained in the carboxyl terminus of GLUT4, a chimera (GTCTR) was constructed in which the carboxyl-terminal 30 amino acids of GLUT4 were substituted for the amino-terminal cytoplasmic domain of the transferrin receptor (TR). The endocytic behavior of this chimera was characterized in Chinese hamster ovary cells. The GTCTR chimera had a more predominant intracellular distribution compared to the TR. Only 20% of the GTCTR chimera is on the surface at steady-state compared to 35% of the TR. The GTCTR chimera is internalized 50% more rapidly and recycled 20% more slowly than the TR. Acidification of the cytosol inhibited internalization of the GTCTR chimera, indicating that the chimera is internalized through clathrin-coated pits. Mutations of GTCTR were constructed in which a di-leucine sequence of the carboxyl domain of GLUT4 was mutated to a di-alanine sequence (GTCTR-AA) and serine residue 488, immediately preceding the di-leucine sequence, was mutated to either an alanine or aspartate residue. In each case, albeit to varying degrees, the substitutions shifted the distribution of the mutated GTCTR constructs toward the surface. The shift in the distribution of GTCTR-AA resulted from a 10-fold reduction in internalization, and the shift of serine 488 mutants resulted from a 3-fold reduction in the internalization rate compared to GTCTR. None of these mutations affected the recycling rate. These results demonstrate that the carboxyl terminus of GLUT4 contains a serine-leucine-leucine-based motif that, when expressed in non-insulin responsive cells, functions as a potent internalization motif which promotes more rapid internalization than does the native TR internalization motif. |
| Databáze: | OpenAIRE |
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