Nucleic Acids Research

Autor: Anne C Barbosa, Zhengyao Xu, Kazhal Karari, Wendi Williams, Silke Hauf, William R A Brown
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Nucleic Acids Research
ISSN: 0305-1048
1362-4962
Popis: We have used chromosome engineering to replace native centromeric DNA with different test sequences at native centromeres in two different strains of the fission yeast Schizosaccharomyces pombe and have discovered that A + T rich DNA, whether synthetic or of bacterial origin, will function as a centromere in this species. Using genome size as a surrogate for the inverse of effective population size (N-e) we also show that the relative A + T content of centromeric DNA scales with N-e across 43 animal, fungal and yeast (Opisthokonta) species. This suggests that in most of these species the A + T content of the centromeric DNA is determined by a balance between selection and mutation. Combining the experimental results and the evolutionary analyses allows us to conclude that A + T rich DNA of almost any sequence will function as a centromere in most Opisthokonta species. The fact that many G/C to A/T substitutions are unlikely to be selected against may contribute to the rapid evolution of centromeric DNA. We also show that a neo-centromere sequence is not simply a weak version of native centromeric DNA and suggest that neo-centromeres require factors either for their propagation or establishment in addition to those required by native centromeres. BBSRC [BB/K003356/1]; Brazilian 'NottinghamBirmingham' PhD scheme by CAPES, Brazil; Kurdistan Regional Government, Human Capacity Development Program; National Institute of General Medical Sciences of the National Institutes of Health [R35GM119723]; University of Nottingham; OUP Published version Nottingham was supported by BBSRC [BB/K003356/1]; A.C.B. was funded by the Brazilian `NottinghamBirmingham' PhD scheme organised by CAPES, Brazil; K.K. was funded by the Kurdistan Regional Government, Human Capacity Development Program; S.H. and W.W. were supported by the National Institute of General Medical Sciences of the National Institutes of Health R35GM119723]. Funding for open access charge: University of Nottingham transformative agreement with OUP.
Databáze: OpenAIRE
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