Sponge Spray-Reaching New Dimensions of Direct Sampling and Analysis by MS
| Autor: | Rynno Lohmus, Kalev Takkis, Karin Kipper, Max Hecht, Koit Herodes, Rudolf Aro, Hanno Evard, Ru Ta Veigure, Ivo Leito |
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| Rok vydání: | 2017 |
| Předmět: |
Electrospray
Blood Specimen Collection Chromatography Chemistry Sample (material) 010401 analytical chemistry Direct sampling Ms analysis Analytical chemistry Sampling (statistics) Analytic Sample Preparation Methods 010402 general chemistry Mass spectrometry 01 natural sciences Mass Spectrometry 0104 chemical sciences Analytical Chemistry Humans Sample preparation Dried Blood Spot Testing Dried blood Urine Specimen Collection |
| Zdroj: | Analytical chemistry. 89(21) |
| ISSN: | 1520-6882 |
| Popis: | Sample preparation for the analysis of clinical samples with the mass spectrometer (MS) can be extensive and expensive. Simplifying and speeding up the process would be very beneficial. This paper reports sponge spray—a novel sampling and direct MS analysis approach—attempting exactly that. It enables direct analysis without any sample preparation from dried blood, plasma, and urine. The tip of a volumetric absorptive microsampling device is used to collect an exact amount of sample and from that same tip an electrospray can be directed into a mass spectrometer. We demonstrate here that, although with significant matrix effects, quantitation of penicillin G, a common antimicrobial, is possible in plasma and in urine, with essentially no sample preparation. |
| Databáze: | OpenAIRE |
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