Real-time imaging of VCAM-1 mRNA in TNF-α activated retinal microvascular endothelial cells using antisense hairpin-DNA functionalized gold nanoparticles
| Autor: | Alexis C. Wong, David W. Wright, John S. Penn, Imam Uddin, Gary W. McCollum, Ashwath Jayagopal |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Cell Survival
Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Metal Nanoparticles Vascular Cell Adhesion Molecule-1 Bioengineering 02 engineering and technology Biology 010402 general chemistry 01 natural sciences Article DNA Antisense Fluorescence chemistry.chemical_compound Mice Animals General Materials Science RNA Messenger VCAM-1 Cells Cultured Messenger RNA Cell adhesion molecule Tumor Necrosis Factor-alpha Retinal Vessels Retinal 021001 nanoscience & nanotechnology Molecular biology 0104 chemical sciences Cell biology Molecular Imaging chemistry Colloidal gold Molecular Medicine Tumor necrosis factor alpha Endothelium Vascular Gold 0210 nano-technology Intracellular DNA |
| Popis: | Vascular cell adhesion molecule 1 (VCAM-1) is an important inflammatory biomarker correlating with retinal disease progression. Thus, detection of VCAM-1 mRNA expression levels at an early disease stage could be an important predictive biomarker to assess the risk of disease progression and monitoring treatment response. We have developed VCAM-1 targeted antisense hairpin DNA-functionalized gold nanoparticles (AS-VCAM-1 hAuNP) for the real time detection of VCAM-1 mRNA expression levels in retinal endothelial cells. The AS-VCAM-1 hAuNP fluorescence enhancement clearly visualized the TNF-α induced cellular VCAM-1 mRNA levels with high signal to noise ratios compared to normal serum treated cells. The scrambled hAuNP probes were minimally detectable under same image acquisition conditions. Intracellular hAuNPs were detected using transmission electron microscopy (TEM) analysis of the intact cells. In addition, the AS-VCAM-1 hAuNP probes exhibited no acute toxicity to the retinal microvascular endothelial cells as measured by live-dead assay. |
| Databáze: | OpenAIRE |
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