Transcriptional Expression of Myelin Basic Protein in Oligodendrocytes Depends on Functional Syntaxin 4: a Potential Correlation with Autocrine Signaling
Autor: | Dick Hoekstra, Jenny C. de Jonge, Wia Baron, Sanjay Tyagi, Marjolein Bijlard, Hans de Vries, Anita Nomden, Bert Klunder |
---|---|
Přispěvatelé: | Molecular Neuroscience and Ageing Research (MOLAR), Nanotechnology and Biophysics in Medicine (NANOBIOMED) |
Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
Proteolipid protein 1
Transcription Genetic CELL POLARITY Primary Cell Culture PELIZAEUS-MERZBACHER-DISEASE Myelin Ganglia Spinal medicine Protein biosynthesis PROTEOLIPID PROTEIN Syntaxin Animals RNA Messenger Rats Wistar Myelin Proteolipid Protein Molecular Biology CULTURED OLIGODENDROCYTES biology Qa-SNARE Proteins CENTRAL-NERVOUS-SYSTEM Gene Expression Regulation Developmental Myelin Basic Protein LOCALIZATION Cell Biology Articles Embryo Mammalian Syntaxin 3 Axons TRANSPORT Myelin proteolipid protein Myelin basic protein Cell biology Rats Autocrine Communication Oligodendroglia medicine.anatomical_structure nervous system Culture Media Conditioned PLASMA-MEMBRANE biology.protein GENE REGULATORY FACTOR Signal transduction MESSENGER-RNA Signal Transduction |
Zdroj: | Molecular and Cellular Biology, 35(4), 675-687. AMER SOC MICROBIOLOGY |
ISSN: | 0270-7306 |
Popis: | Myelination of axons by oligodendrocytes is essential for saltatory nerve conduction. To form myelin membranes, a coordinated synthesis and subsequent polarized transport of myelin components are necessary. Here, we show that as part of the mechanism to establish membrane polarity, oligodendrocytes exploit a polarized distribution of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) machinery components syntaxins 3 and 4, localizing to the cell body and the myelin membrane, respectively. Our data further reveal that the expression of myelin basic protein (MBP), a myelin-specific protein that is synthesized "on site" after transport of its mRNA, depends on the correct functioning of the SNARE machinery, which is not required for mRNA granule assembly and transport per se. Thus, downregulation and overexpression of syntaxin 4 but not syntaxin 3 in oligodendrocyte progenitor cells but not immature oligodendrocytes impeded MBP mRNA transcription, thereby preventing MBP protein synthesis. The expression and localization of another myelin-specific protein, proteolipid protein (PLP), was unaltered. Strikingly, conditioned medium obtained from developing oligodendrocytes was able to rescue the block of MBP mRNA transcription in syntaxin 4-downregulated cells. These findings indicate that the initiation of the biosynthesis of MBP mRNA relies on a syntaxin 4-dependent mechanism, which likely involves activation of an autocrine signaling pathway. |
Databáze: | OpenAIRE |
Externí odkaz: |