The association of two single nucleotide polymorphisms (SNPs) in growth hormone (GH) gene with litter size and superovulation response in goat-breeds
| Autor: | Kunkun Huang, Chunyan Zhang, Wenbing Zeng, Qunying Wen, Liguo Yang, Yun Liu, Deqing Xu |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Litter (animal)
medicine.medical_specialty lcsh:QH426-470 Single-nucleotide polymorphism Biology medicine.disease_cause Growth hormone Andrology Internal medicine Genotype Genetics medicine Molecular Biology Gene Mutation superovulation response goat Breed Lower incidence lcsh:Genetics Endocrinology DNA polymorphism growth hormone (GH) gene litter size Animal Genetics Research Article |
| Zdroj: | Genetics and Molecular Biology v.34 n.1 2011 Genetics and Molecular Biology Sociedade Brasileira de Genética (SBG) instacron:SBG Genetics and Molecular Biology, Volume: 34, Issue: 1, Pages: 49-55, Published: 22 DEC 2010 Genetics and Molecular Biology, Vol 34, Iss 1, Pp 49-55 (2011) |
| Popis: | Two active mutations (A 781 G and A 1575 G) in growth hormone (GH) gene, and their associations with litter size (LS), were investigated in both a high prolificacy (Matou, n = 182) and a low prolificacy breed (Boer, n = 352) by using the PCR-RFLP method. Superovulation experiments were designed in 57 dams, in order to evaluate the effect of different genotypes of the GH gene on superovulation response. Two genotypes (AA and AB, CC and CD) in each mutation were detected in these two goat breeds. Neither BB nor DD homozygous genotypes were observed. The genotypic frequencies of AB and CC were significantly higher than those of AA and CD. In the third parity, Matou dams with AB or CC genotypes had significantly larger litter sizes than those with AA and CD (p < 0.05). On combining the two loci, both Matou and Boer dams with ABCD genotype had the largest litter sizes when compared to the other genotypes (p < 0.05). When undergoing like superovulation treatments, a significantly higher number of corpora lutea and ova, with a lower incidence of ovarian cysts, were harvested in the AB and CC genotypes than in AA and CD. These results show that the two loci of GH gene are highly associated with abundant prolificacy and superovulation response in goat breeds. |
| Databáze: | OpenAIRE |
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