ADP-Ribosylation of Brain Neuronal Proteins Is Altered by In Vitro and In Vivo Exposure to Inorganic Mercury
| Autor: | Fritz L. Lorscheider, Henk Zwiers, Pawel Palkiewicz |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
Nerve Tissue Proteins Biochemistry Rats Sprague-Dawley Cellular and Molecular Neuroscience Neurochemical In vivo Animals Electrophoresis Gel Two-Dimensional Cytoskeleton Actin Neurons Adenosine Diphosphate Ribose biology In vitro Frontal Lobe Rats Cell biology Molecular Weight Tubulin ADP-ribosylation Phosphoprotein Mercuric Chloride biology.protein Autoradiography Phosphorus Radioisotopes |
| Zdroj: | Journal of Neurochemistry. 62:2049-2052 |
| ISSN: | 1471-4159 0022-3042 |
| Popis: | ADP-ribosylation is an essential prooess in the metabolism of brain neuronal proteins, including the regulation of assembly and disassembly of biological polymers. Here, we examine the effect of HgCl, exposure on the ADP-ribosylation of tubulin and actin, both cytoskeletal proteins also found in neurons, and B-50/ 43-kDa growth-associated protein (B-50/GAP-43), a neuronal tissue-specific phosphoprotein. In rats we demonstrate, with both in vitro and in vivo experiments, that HgCl 2 markedly inhibits the ADP-ribosylation of tubulin and actin. This is direct quantitative evidence that HgCl 2 , a toxic xenobiotic, alters specific neurochemical reactions involved in maintaining brain neuron structure |
| Databáze: | OpenAIRE |
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