Nitric oxide activates an Nrf2/sulfiredoxin antioxidant pathway in macrophages

Autor: Jean-Claude Drapier, Michel B. Toledano, Jacques Breton, Jérôme Bignon, Anne-Gaëlle Planson, Cécile Bouton, Kahina Abbas, Cendrine Seguin, Sylvie Riquier
Přispěvatelé: Institut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), French National Agency [06-blan-0277-01], Stress oxydatif et risque cardiovasculaire : interaction fondamentale et clinique (U866, Cancer, équipe 4), Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Institut Pasteur Centre National de la Recherche Scientifique (CNRS)Pasteur-Weizmann program Région Ile-de-France
Rok vydání: 2011
Předmět:
Antioxidant
[SDV]Life Sciences [q-bio]
medicine.medical_treatment
medicine.disease_cause
Polymerase Chain Reaction
Biochemistry
Antioxidants
Mice
chemistry.chemical_compound
0302 clinical medicine
Oxidoreductases Acting on Sulfur Group Donors
Cells
Cultured
Mice
Knockout
chemistry.chemical_classification
0303 health sciences
[CHIM.ORGA]Chemical Sciences/Organic chemistry
MALTOSE-BINDING PROTEIN
3. Good health
Cell biology
ESCHERICHIA-COLI
NMR-SPECTROSCOPY
Oxidation-Reduction
Peroxynitrite
NF-E2-Related Factor 2
ANTIGEN
IMMUNITY
Biology
Nitric Oxide
Nitric oxide
03 medical and health sciences
Physiology (medical)
parasitic diseases
medicine
Animals
MALARIA VACCINE CANDIDATE
PARASITE
Transcription factor
030304 developmental biology
Reactive oxygen species
COMPLEX
IDENTIFICATION
Macrophages
Hydrogen Peroxide
Immunity
Innate
Mice
Inbred C57BL
Oxidative Stress
Sulfiredoxin
chemistry
ANTIBODIES
Reactive Oxygen Species
030217 neurology & neurosurgery
Oxidative stress
Cysteine
Zdroj: Free Radical Biology and Medicine
Free Radical Biology and Medicine, Elsevier, 2011, 51 (1), pp.107-114. ⟨10.1016/j.freeradbiomed.2011.03.039⟩
ISSN: 0891-5849
DOI: 10.1016/j.freeradbiomed.2011.03.039
Popis: International audience; Malaria represents a major public health problem and an important cause of mortality and morbidity. The malaria parasites are becoming resistant to drugs used to treat the disease and still no efficient vaccine has been developed. One promising vaccine candidate is the merozoite surface protein 1 (MSP1), which has been extensively investigated as a vaccine target. The surface protein MSP1 plays an essential role in the erythrocyte invasion process and is an accessible target for the immune system. Antibodies to the carboxy-terminal region of the protein, named MSP1(19), can inhibit erythrocyte invasion and parasite growth. In order to develop an effective MSP1(19)- based vaccine against malaria, production of an antigen that is recognized by protective antibodies is mandatory. To this aim, we propose a method to produce the disulfide-rich MSP1(19) in its native conformation based on its in vitro oxidative refolding. The native conformation of the renatured MSP1(19) is carefully established by immunochemical reactivity experiments, circular dichroism and NMR. MSP1(19) can successfully be refolded in vitro as an isolated protein or as a fusion with the maltose binding protein. The possibility to properly fold MSP1(19) in vitro paves the way to new approaches for high titer production of native MSP1(19) using Escherichia coli as a host.
Databáze: OpenAIRE
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