Binding of norgestrel to human plasma proteins
| Autor: | Kesho R. Laumas, J.P. Uniyal |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Male
Protein Conformation Plasma protein binding Binding Competitive Biochemistry Genetics and Molecular Biology (miscellaneous) Pregnancy Norgestrel Centrifugation Density Gradient medicine Humans Serum Albumin Binding Sites Chromatography biology Chemistry Binding protein Blood Proteins Hydrogen-Ion Concentration Ligand (biochemistry) Human serum albumin Blood proteins Molecular Weight Dissociation constant Biochemistry biology.protein Female Protein G Dialysis Protein Binding medicine.drug |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Protein Structure. 427:218-230 |
| ISSN: | 0005-2795 |
| DOI: | 10.1016/0005-2795(76)90298-1 |
| Popis: | Binding of [14,15- 3 H ](±)- norgestrel to human plasma proteins has been investigated. Norgestrel showed greater affinity to plasma than to human serum albumin indicating specific norgestrel binding protein(s) in the plasma. α 1 - acid glycoprotein showed high affinity for norgestrel when compared with human serum albumin. The binding protein was eluted at pH 5.8 by step by step elution on a DEAE-cellulose column. Norgestrel binding to plasma proteins was not affected at 60 °C. The optimal binding occurred between pH 7 and 8. Ligand specificity of the binding protein revealed that progesterone was able to compete for the norgestrel binding sites, whereas corticosterone, testosterone, oestradiol, and norethindrone acetate did not show much competition. The molecular weight of the binding protein was found to be approximately 43 000. Sucrose density gradient analysis indicated that norgestrel bound to a macromolecular component of sedimentation coefficient 2.9 S. The association constant ( K ass ) and dissociation constant ( K diss ) of norgestrel-binding plasma protein was found to be 1.4·106 M−1 and 0.7·10−6M respectively. The number of binding sites was 0.5·10−9 mol/mg protein. Norgestrel-binding protein in the plasma appeared to be a protein different from human serum albumin, corticosteroid-binding globulin and sex-steroid-binding protein. This binding protein showed some similarities to α 1 - acid glycoprotein. |
| Databáze: | OpenAIRE |
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