Suppression of the lipopolysaccharide-induced expression of MARCKS-related protein (MRP) affects transmigration in activated RAW264.7 cells
| Autor: | Kyoungho Suk, Eun Mi Bae, Won-Ha Lee, Kwang-Rok Chun, Jae-Kwan Kim |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Lipopolysaccharides
Immunology Down-Regulation Gene Expression Biology Transfection Cell Line Small hairpin RNA Mice Phagocytosis stomatognathic system Downregulation and upregulation Animals Macrophage RNA Small Interfering MARCKS Chemokine CCL2 Protein kinase C DNA Primers Base Sequence Chemotaxis Macrophages Microfilament Proteins Intracellular Signaling Peptides and Proteins Macrophage Activation Molecular biology Cell biology Matrix Metalloproteinase 9 Cell culture Calmodulin-Binding Proteins |
| Zdroj: | Cellular Immunology. 256:92-98 |
| ISSN: | 0008-8749 |
| Popis: | The molecular action mechanism of MRP, one of the protein kinase C (PKC) substrates, has been under intense investigation, but reports on its role in macrophage function remain controversial. The treatment of macrophage cell lines with bacterial lipopolysaccharide (LPS) induced a high level of MRP expression suggesting that MRP plays a role in the function of activated macrophages. In order to investigate the role of MRP in activated RAW264.7 cells, we stably transfected MRP-specific shRNA expression constructs and tested for alterations in macrophage-related functions. The down-regulation of MRP expression resulted in a marked reduction in chemotaxis toward MCP-1 or extracellular matrix proteins. Furthermore, pharmacological inhibitors of PKC significantly inhibited the chemotaxis in RAW264.7 cells. These data reveals the pivotal role of MRP in the transmigration of activated RAW264.7 cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect