Mycobacteria Clumping Increase Their Capacity to Damage Macrophages
| Autor: | Míriam Pérez-Trujillo, Fernando Alcaide, Cristina Tomàs-Martínez, Marta Llorens-Fons, Thomas F. Byrd, Esther Julián, Estela Noguera-Ortega, Marina Luquin, Cecilia Brambilla |
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| Přispěvatelé: | Universitat de Barcelona |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Bacilli Infection of macrophages Macròfags Tuberculosi Phagocytosis 030106 microbiology lcsh:QR1-502 virulence factors Smooth morphotypes Virulence Mycobacterium abscessus Microbiology lcsh:Microbiology Virulència (Microbiologia) Rough morphotypes Proinflammatory cytokine law.invention Virulence (Microbiology) Mycobacterium tuberculosis 03 medical and health sciences law Citoquines Tuberculosis infection of macrophages Cords Original Research clumps smooth morphotypes Virulence factors biology Macrophages Vesicle rough morphotypes biology.organism_classification 030104 developmental biology Cytokines cords Clumps Electron microscope |
| Zdroj: | Dipòsit Digital de la UB Universidad de Barcelona Frontiers in Microbiology Frontiers in Microbiology, Vol 7 (2016) Recercat. Dipósit de la Recerca de Catalunya instname Recercat: Dipósit de la Recerca de Catalunya Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2016.01562 |
| Popis: | The rough morphotypes of non-tuberculous mycobacteria have been associated with the most severe illnesses in humans. This idea is consistent with the fact that Mycobacterium tuberculosis presents a stable rough morphotype. Unlike smooth morphotypes, the bacilli of rough morphotypes grow close together, leaving no spaces among them and forming large aggregates (clumps). Currently, the initial interaction of macrophages with clumps remains unclear. Thus, we infected J774 macrophages with bacterial suspensions of rough morphotypes of Mycobacterium abscessus containing clumps and suspensions of smooth morphotypes, primarily containing isolated bacilli. Using confocal laser scanning microscopy and electron microscopy, we observed clumps of at least 5 rough-morphotype bacilli inside the phagocytic vesicles of macrophages at 3 hours post-infection. These clumps grew within the phagocytic vesicles, killing 100% of the macrophages at 72 hours post-infection, whereas the proliferation of macrophages infected with smooth morphotypes remained unaltered at 96 hours post-infection. Thus, macrophages phagocytose large clumps, exceeding the bactericidal capacities of these cells. Furthermore, proinflammatory cytokines and granuloma-like structures were only produced by macrophages infected with rough morphotypes. Thus, the present study provides a foundation for further studies that consider mycobacterial clumps as virulence factors. |
| Databáze: | OpenAIRE |
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