Tet-On Systems For Doxycycline-inducible Gene Expression
| Autor: | Liliane Tenenbaum, Ben Berkhout, Atze T. Das |
|---|---|
| Přispěvatelé: | AII - Amsterdam institute for Infection and Immunity, Medical Microbiology and Infection Prevention |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Operon Genetic enhancement Biology TetR Transfection Vaccines Attenuated Article 03 medical and health sciences Bacterial Proteins Drug Discovery Gene expression Genetics medicine polycyclic compounds Escherichia coli Gene silencing Humans Promoter Regions Genetic Molecular Biology Gene Tet-On system tTA Genetics (clinical) Doxycycline AIDS Vaccines Transcription regulation Genetic Therapy Tetracycline Molecular biology 3. Good health 030104 developmental biology Gene Expression Regulation Tet-Off system HIV-1 Molecular Medicine Carrier Proteins Function (biology) medicine.drug rtTA |
| Zdroj: | Current Gene Therapy Current gene therapy, 16(3), 156-167. Bentham Science Publishers B.V. Europe PubMed Central |
| ISSN: | 1875-5631 1566-5232 |
| Popis: | The tetracycline-controlled Tet-Off and Tet-On gene expression systems are used to regulate the activity of genes in eukaryotic cells in diverse settings, varying from basic biological research to biotechnology and gene therapy applications. These systems are based on regulatory elements that control the activity of the tetracycline-resistance operon in bacteria. The Tet-Off system allows silencing of gene expression by administration of tetracycline (Tc) or tetracycline-derivatives like doxycycline (dox), whereas the Tet-On system allows activation of gene expression by dox. Since the initial design and construction of the original Tet-system, these bacterium-derived systems have been significantly improved for their function in eukaryotic cells. We here review how a dox-controlled HIV-1 variant was designed and used to greatly improve the activity and dox-sensitivity of the rtTA transcriptional activator component of the Tet-On system. These optimized rtTA variants require less dox for activation, which will reduce side effects and allow gene control in tissues where a relatively low dox level can be reached, such as the brain. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|