Platelet Mitochondrial Respiration, Endogenous Coenzyme Q10 and Oxidative Stress in Patients with Chronic Kidney Disease
| Autor: | Zuzana Sumbalová, Zuzana Rausová, O Vancova, Mária Komlósi, Anna Gvozdjáková, Viliam Mojto, Jarmila Kucharská, Monika Szamosová |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Clinical Biochemistry Renal function 030204 cardiovascular system & hematology Mitochondrion medicine.disease_cause urologic and male genital diseases Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine coenzyme Q10 Internal medicine Diabetes mellitus medicine TBARS oxidative stress Platelet Coenzyme Q10 lcsh:R5-920 business.industry medicine.disease mitochondria 030104 developmental biology Endocrinology chemistry platelets business lcsh:Medicine (General) Oxidative stress respiration chronic kidney disease Kidney disease |
| Zdroj: | Diagnostics Volume 10 Issue 3 Diagnostics, Vol 10, Iss 3, p 176 (2020) |
| ISSN: | 2075-4418 |
| DOI: | 10.3390/diagnostics10030176 |
| Popis: | Chronic kidney disease (CKD) is characterized by a progressive loss of renal function and a decrease of glomerular filtration rate. Reduced mitochondrial function, coenzyme Q10 (CoQ10), and increased oxidative stress in patients with CKD contribute to the disease progression. We tested whether CoQ10 levels, oxidative stress and platelet mitochondrial bioenergetic function differ between groups of CKD patients. Methods: Twenty-seven CKD patients were enrolled in this trial, 17 patients had arterial hypertension (AH) and 10 patients had arterial hypertension and diabetes mellitus (AH and DM). The control group consisted of 12 volunteers. A high-resolution respirometry (HRR) method was used for the analysis of mitochondrial bioenergetics in platelets, and an HPLC method with UV detection was used for CoQ10 determination in platelets, blood, and plasma. Oxidative stress was determined as thiobarbituric acid reactive substances (TBARS). Results: Platelets mitochondrial respiration showed slight, not significant differences between the groups of CKD patients and control subjects. The oxygen consumption by intact platelets positively correlated with the concentration of CoQ10 in the platelets of CKD patients. Conclusion: A decreased concentration of CoQ10 and oxidative stress could contribute to the progression of renal dysfunction in CKD patients. The parameters of platelet respiration assessed by high-resolution respirometry can be used only as a weak biological marker for mitochondrial diagnosis and therapy monitoring in CKD patients. |
| Databáze: | OpenAIRE |
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