Genome-Wide SNP-Genotyping Array to Study the Evolution of the Human Pathogen Vibrio vulnificus Biotype 3
| Autor: | Yudi Bar-On, Yechezkel Kashi, Nili Raz, David R. Walt, Eva Sanjuán, Alex Linetsky, Ryan B. Hayman, Carmen Amaro, Yael Danin-Poleg, Michael Shmoish |
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| Rok vydání: | 2014 |
| Předmět: |
Genotyping
Genome evolution lcsh:Medicine Marine and Aquatic Sciences Genome Viral Vibrio vulnificus Polymorphism Single Nucleotide Microbiology Genome Evolution Molecular Molecular Genetics Genetics lcsh:Science Molecular Biology Techniques Clade Molecular Biology Comparative genomics Evolutionary Biology Bacterial Evolution Multidisciplinary biology Phylogenetic tree lcsh:R Ecology and Environmental Sciences Biology and Life Sciences Aquatic Environments biology.organism_classification Organismal Evolution SNP genotyping Haplotypes Bacteris patògens Microbial Evolution Earth Sciences lcsh:Q Population Genetics Research Article |
| Zdroj: | PLoS ONE Raz, Nili Danin-Poleg, Yael Hayman, Ryan B. Bar-On, Yudi Linetsky, Alex Shmoish, Michael Sanjuán Caro, Eva Amaro González, Carmen Walt, David R. Kashi, Yechezkel 2014 Genome-Wide SNP-genotyping array to study the evolution of the human pathogen Vibrio vulnificus Biotype 3 Plos One 9 12 1 21 RODERIC. Repositorio Institucional de la Universitat de Valéncia instname PLoS ONE, Vol 9, Iss 12, p e114576 (2014) |
| ISSN: | 1932-6203 |
| Popis: | Vibrio vulnificus is an aquatic bacterium and an important human pathogen. Strains Of V. vulnificus are classified into three different biotypes. The newly emerged biotype 3 has been found to be clonal and restricted to Israel. In the family Vibrionaceae , horizontal gene transfer is the main mechanism responsible for the emergence of new pathogen groups. To better understand the evolution of the bacterium, and in particular to trace the evolution of biotype 3, we performed genome-wide SNP genotyping of 254 clinical and environmental V. vulnificus isolates with worldwide distribution recovered over a 30-year period, representing all phylogeny groups. A custom single-nucleotide polymorphism (SNP) array implemented on the Illumina GoldenGate platform was developed based on 570 SNPs randomly distributed throughout the genome. In general, the genotyping results divided the V. vulnificus species into three main phylogenetic lineages and an additional subgroup, clade B, consisting of environmental and clinical isolates from Israel. Data analysis suggested that 69% of biotype 3 SNPs are similar to SNPs from clade B, indicating that biotype 3 and clade B have a common ancestor. The rest of the biotype 3 SNPs were scattered along the biotype 3 genome, probably representing multiple chromosomal segments that may have been horizontally inserted into the clade B recipient core genome from other phylogroups or bacterial species sharing the same ecological niche. Results emphasize the continuous evolution of V. vulnificus and support the emergence of new pathogenic groups within this species as a recurrent phenomenon. Our findings contribute to a broader understanding of the evolution of this human pathogen. |
| Databáze: | OpenAIRE |
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