Large depth-of-field tracking of colloidal spheres in holographic microscopy by modeling the objective lens
| Autor: | Solomon Barkley, Caroline Martin, Vinothan N. Manoharan, Brian Leahy, Ronald Alexander |
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| Rok vydání: | 2019 |
| Předmět: |
Materials science
Microscope business.industry Holography FOS: Physical sciences 02 engineering and technology 021001 nanoscience & nanotechnology Tracking (particle physics) 01 natural sciences Atomic and Molecular Physics and Optics law.invention 010309 optics Lens (optics) Optics Cardinal point law 0103 physical sciences Microscopy Particle Depth of field 0210 nano-technology business Physics - Optics Optics (physics.optics) |
| DOI: | 10.48550/arxiv.1910.14493 |
| Popis: | Holographic microscopy has developed into a powerful tool for 3D particle tracking, yielding nanometer-scale precision at high frame rates. However, current particle tracking algorithms ignore the effect of the microscope objective on the formation of the recorded hologram. As a result, particle tracking in holographic microscopy is currently limited to particles well above the microscope focus. Here, we show that modeling the effect of an aberration-free lens allows tracking of particles above, near, and below the focal plane in holographic microscopy, doubling the depth of field. Finally, we use our model to determine the conditions under which ignoring the effect of the lens is justified and in what conditions it leads to systematic errors. |
| Databáze: | OpenAIRE |
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