The insecticide buprofezin induces morphological transformation and kinetochore-positive micronuclei in cultured Syrian hamster embryo cells in the absence of detectable DNA damage
| Autor: | Q. Y. Chen, Patricia Ostrosky-Wegman, Dietmar Schiffmann, Luis A. Herrera, U. Andrae, K. Ziegler-Skylakakis |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Insecticides
DNA repair DNA damage Centromere Hamster Biology medicine.disease_cause Microtubules Cricetinae medicine Animals Cells Cultured Genetics Micronucleus Tests Mesocricetus Molecular Structure Thiadiazines Embryo General Medicine Molecular biology Cell Transformation Neoplastic Cell culture Micronucleus test Genotoxicity Immunostaining DNA Damage Mutagens |
| Zdroj: | Mutation research. 303(3) |
| ISSN: | 0027-5107 |
| Popis: | The insecticide buprofezin was examined for its genotoxicity in cultured Syrian hamster embryo cells in order to better understand the mechanisms underlying the genotoxicity of the compound in mammalian cells. Exposure to buprofezin concentrations of 12.5–100 μM did not significantly affect the colony-forming ability of the cells, but did result in increased frequencies of morphologically transformed colonies. Treatment with buprofezin did not cause a detectable induction of DNA repair synthesis, an indicator of DNA damage, but significantly increased the frequency of micronuclei. Immunostaining of the cells with antikinetochore antibody (CREST antibody) showed that essentially all of the buprofezin-induced micronuclei were kinetochore-positive. The results suggest that morphological transformation of Syrian hamster embryo cells by buprofezin results from an interaction of the compound or a metabolite of it with the mitotic apparatus rather than from DNA damage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|