Candida Species From Eye Infections: Drug Susceptibility, Virulence Factors, and Molecular Characterization
| Autor: | Kanchana Chathoth, Savitri Sharma, Konduri Ranjith, Kalyana C. Sama, Joveeta Joseph, Somasheila I Murthy, Bhavani Sontam, Sisinthy Shivaji |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Male 0301 basic medicine Antifungal Agents Natamycin Virulence Factors 030106 microbiology Virulence Microbial Sensitivity Tests Candida parapsilosis Microbiology Young Adult 03 medical and health sciences Minimum inhibitory concentration 0302 clinical medicine Amphotericin B Genotype medicine Humans Aged Candida Aged 80 and over Keratitis Endophthalmitis biology Candidiasis RNA Fungal Sequence Analysis DNA Middle Aged Orbital Cellulitis Eye infection biology.organism_classification RNA Ribosomal Biofilms Child Preschool 030221 ophthalmology & optometry Female Coagulase Eye Infections Fungal medicine.drug |
| Zdroj: | Investigative Opthalmology & Visual Science. 58:4201 |
| ISSN: | 1552-5783 |
| DOI: | 10.1167/iovs.17-22003 |
| Popis: | Purpose To determine the type of Candida species in ocular infections and to investigate the relationship of antifungal susceptibility profile to virulence factors. Methods Fifty isolates of yeast-like fungi from patients with keratitis, endophthalmitis, and orbital cellulitis were identified by Vitek-2 compact system and DNA sequencing of ITS1-5.8S-ITS2 regions of the rRNA gene, followed by phylogenetic analysis for phenotypic and genotypic identification, respectively. Minimum inhibitory concentration of six antifungal drugs was determined by E test/microbroth dilution methods. Phenotypic and genotypic methods were used to determine the virulence factors. Results Phylogenetic analysis showed the clustering of all isolates into eight distinct groups with a major cluster formed Candida parapsilosis (n = 21), which was the most common species by both Vitek 2 and DNA sequencing. Using χ2 test no significant difference was noted between the techniques except that Vitek 2 did not identify C. viswanathii, C. orthopsilosis, and two non-Candida genera. Of 43 tested Candida isolates high susceptibility to amphotericin B (39/43, 90.6%) and natamycin (43/43, 100%) was noted. While none of the isolates produced coagulase, all produced esterase and catalase. The potential to form biofilm was detected in 23/43 (53.4%) isolates. Distribution of virulence factors by heat map analysis showed difference in metabolic activity of biofilm producers from nonbiofilm producers. Conclusions Identified by Vitek 2 and DNA sequencing methods C. parapsilosis was the most common species associated with eye infections. Irrespective of the virulence factors elaborated, the Candida isolates were susceptible to commonly used antifungal drugs such as amphotericin B and natamycin. |
| Databáze: | OpenAIRE |
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