Microbiome analysis of Ixodes scapularis ticks from New York and Connecticut
| Autor: | Xiaoyu Che, Teresa Tagliafierro, Komal Jain, W. Ian Lipkin, Rafal Tokarz, Alexandra Oleynik, Stephen Sameroff, D. Moses Cucura |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Nymph 0301 basic medicine Nematoda animal diseases 030231 tropical medicine New York Borrelia miyamotoi Tick Babesia microti Microbiology Encephalitis Viruses Tick-Borne 03 medical and health sciences 0302 clinical medicine Borrelia mayonii Borrelia parasitic diseases Animals Powassan virus Rickettsia Borrelia burgdorferi Ehrlichia muris Bacteria Ixodes biology Microbiota High-Throughput Nucleotide Sequencing bacterial infections and mycoses biology.organism_classification Virology Connecticut 030104 developmental biology Infectious Diseases Ixodes scapularis Insect Science Viruses bacteria Female Parasitology Metagenomics Anaplasma phagocytophilum |
| Zdroj: | Ticks and Tick-borne Diseases. 10:894-900 |
| ISSN: | 1877-959X |
| Popis: | We employed high throughput sequencing to survey the microbiomes of Ixodes scapularis collected in New York and Connecticut. We examined 197 individual I. scapularis adults and pools from 132 adults and 197 nymphs. We detected Borrelia burgdorferi sensu stricto in 56.3% of individual ticks, Anaplasma phagocytophilum in 10.6%, Borrelia miyamotoi in 5%, Babesia microti in 7.6%, and Powassan virus in 3.6%. We did not detect Borrelia mayonii, Ehrlichia muris eauclairensis, Bartonella spp. or pathogenic Babesia species other than B. microti. The most abundant bacterium (65%), and only rickettsial species identified, was the endosymbiont Rickettsia buchneri. A filarial nematode was found in 13.7% of adult ticks. Fourteen viruses were detected including South Bay virus (22%) and blacklegged tick phlebovirus 1 and 2 (73%). This study provides insight into the microbial diversity of I. scapularis in New York State and Connecticut. |
| Databáze: | OpenAIRE |
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