Toxicological effects of graphene oxide on adult zebrafish (Danio rerio)
| Autor: | Jéssica F. Baretta, Valtencir Zucolotto, Fabrício A. Santos, Jaqueline Pérola Souza, Iêda Maria Martinez Paino |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Gills
Male Gill animal structures Necrosis Health Toxicology and Mutagenesis Static Electricity Danio Apoptosis 02 engineering and technology Vacuole 010501 environmental sciences Aquatic Science Microscopy Atomic Force medicine.disease_cause 01 natural sciences Toxicity Tests medicine Animals Zebrafish 0105 earth and related environmental sciences chemistry.chemical_classification Reactive oxygen species biology Chemistry Anatomy 021001 nanoscience & nanotechnology biology.organism_classification Cell biology Liver Nanoparticles CARBONO Female Graphite medicine.symptom Reactive Oxygen Species 0210 nano-technology Water Pollutants Chemical Genotoxicity Oxidative stress DNA Damage Mutagens |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0166-445X |
| Popis: | Graphene exhibits unique physical and chemical properties that facilitate its application in many fields, including electronics and biomedical areas. However, the use of graphene and its derivatives could result in accumulation in aquatic environments, and the risks posed by these compounds for organisms are not completely understood. In this study, we investigated the effects of graphene oxide (GO) on adult zebrafish (Danio rerio). Experimental fish were exposed to 2, 10 or 20mgL-1 GO, and the cytotoxicity, genotoxicity and oxidative stress were assessed. The morphology of the gills and liver tissues was also analyzed. Graphene oxide exposure led to an increase in the number of gill cells that were in early apoptotic and necrotic stages, but genotoxicity was not observed in blood cells. We also observed the generation of Reactive Oxygen Species (ROS) in gill cells. Structural analysis revealed injuries to gill tissues, including a dilated marginal channel, lamellar fusion, clubbed tips, swollen mucocytes, epithelial lifting, aneurysms, and necrosis. Liver tissues also presented lesions such as peripherally located nuclei. Furthermore, hepatocytes exhibited a non-uniform shape, picnotic nuclei, vacuole formation, cell rupture, and necrosis. Our results showed that sub-lethal doses of graphene oxide could be harmful to fish species and thus represent risks for the aquatic food chain. |
| Databáze: | OpenAIRE |
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