3D reconstitution of nerve-blood vessel networks using skeletal muscle-derived multipotent stem cell sheet pellets
| Autor: | Nobuyuki Nakajima, Tetsuro Tamaki, Akihiro Sakai, Yoshiyasu Uchiyama, Hiroyuki Hashimoto, Kosuke Saito, Shuichi Soeda, Nahoko Fukunishi, Toshiro Terachi, Maki Masuda, Joji Mochida |
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| Rok vydání: | 2013 |
| Předmět: |
Embryology
Cellular differentiation Blotting Western Green Fluorescent Proteins Biomedical Engineering Schwann cell Mice Transgenic Biology Real-Time Polymerase Chain Reaction Immunoenzyme Techniques Mice medicine Animals Peripheral Nerves RNA Messenger Muscle Skeletal Cells Cultured Cell Proliferation Tissue Engineering Tissue Scaffolds Reverse Transcriptase Polymerase Chain Reaction Multipotent Stem Cells Skeletal muscle Cell Differentiation Cell biology Nerve Regeneration Transplantation Endothelial stem cell Mice Inbred C57BL medicine.anatomical_structure Multipotent Stem Cell Immunology Blood Vessels Pericyte Endoneurium Biomarkers Stem Cell Transplantation |
| Zdroj: | Regenerative medicine. 8(4) |
| ISSN: | 1746-076X |
| Popis: | Aim: To cover the large tissue deficits associated with significant loss of function following surgery, a 3D gel-patch-like nerve–vascular reconstitution system was developed using the skeletal muscle-derived multipotent stem cell (Sk-MSC) sheet pellet. Materials & methods: The Sk-MSC sheet pellet was prepared from GFP transgenic mice by the collagenase extraction and 7 days expansion cell culture, and transplanted into a severe muscle damage model with large disruptions to muscle fibers, blood vessels and peripheral nerves. Results: At 4 weeks after transplantation, engrafted cells contributed to nerve–vascular regeneration associated with cellular differentiation into Schwann cells, perineurial/endoneurial cells, vascular endothelial cells and pericytes. However, skeletal myogenic differentiation was scarcely observed. Paracrine effects regarding donor cells/tissues could also be expected, because of the active expression of neurogenic and vasculogenic factor mRNAs in the sheet pellet. Conclusion: These results indicate that the vigorous skeletal myogenic potential of Sk-MSCs was clearly reduced in the sheet pellet preparation and this method may be a useful adjuvant for nerve–vascular regeneration in various tissue engineering applications. |
| Databáze: | OpenAIRE |
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