Cell-Type-Specific Quantification of a Scaffold-Based 3D Liver Co-Culture
| Autor: | Marina Häcker, Vanessa Kieber, Victor Häussling, Leon Olde Damink, Andreas K. Nussler, Caren Linnemann, Sabrina Ehnert, Marc Ruoß, Helen Rinderknecht, Silas Rebholz |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Scaffold Cell type cell number QH301-705.5 Cell type specific Matrix (biology) Biochemistry Genetics and Molecular Biology (miscellaneous) Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine pcr-based method Structural Biology Biology (General) Chemistry Resazurin co-culture quantification Cell biology 030104 developmental biology Cell culture 030220 oncology & carcinogenesis Hepg2 cells Co-Culture 3d culture Biotechnology |
| Zdroj: | Methods and Protocols, Vol 3, Iss 1, p 1 (2019) Methods and Protocols Volume 3 Issue 1 |
| ISSN: | 2409-9279 |
| Popis: | In order to increase the metabolic activity of human hepatocytes and liver cancer cell lines, many approaches have been reported in recent years. The metabolic activity could be increased mainly by cultivating the cells in 3D systems or co-cultures (with other cell lines). However, if the system becomes more complex, it gets more difficult to quantify the number of cells (e.g., on a 3D matrix). Until now, it has been impossible to quantify different cell types individually in 3D co-culture systems. Therefore, we developed a PCR-based method that allows the quantification of HepG2 cells and 3T3-J2 cells separately in a 3D scaffold culture. Moreover, our results show that this method allows better comparability between 2D and 3D cultures in comparison to the often-used approaches based on metabolic activity measurements, such as the conversion of resazurin. |
| Databáze: | OpenAIRE |
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