Assignment of Staphylococcus Isolates to Groups by spa Typing, SmaI Macrorestriction Analysis, and Multilocus Sequence Typing
| Autor: | Birgit Strommenger, Christiane Kettlitz, Dag Harmsen, Alexander W. Friedrich, Thomas Weniger, Wolfgang Witte |
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| Rok vydání: | 2006 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Staphylococcus aureus Genotype Sequence analysis Computational biology Biology Staphylococcal infections Microbiology SmaI medicine Cluster Analysis Humans Typing Deoxyribonucleases Type II Site-Specific Staphylococcal Protein A Cluster analysis Molecular Epidemiology Molecular epidemiology Bacteriology Sequence Analysis DNA Staphylococcal Infections medicine.disease DNA Fingerprinting Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field DNA profiling Multilocus sequence typing Polymorphism Restriction Fragment Length |
| Zdroj: | Journal of Clinical Microbiology. 44:2533-2540 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | The implementation of the new clustering algorithm B ased U pon R epeat P attern (BURP) into the Ridom StaphType software tool enables clustering based on spa typing data for Staphylococcus aureus . We compared clustering results obtained by spa typing/BURP to those obtained by currently well-established methods, i.e., SmaI macrorestriction analysis and multilocus sequence typing/eBURST. A total of 99 clinical S. aureus strains, including MRSA and representing major clonal lineages associated with important kinds of infections which have been prevalent in Germany and Central Europe during the last 10 years, were used for comparison. SmaI macrorestriction analysis revealed the highest discriminatory power, and clustering results for all three methods resulted in concordance values ranging from 96.8% between the two sequence-based methods to 93.4% between spa typing/BURP and SmaI macrorestriction/cluster analysis. The results of this study indicate that spa typing, together with BURP clustering, is a useful tool in S. aureus epidemiology, especially because of ease of use and the advantages of unambiguous sequence analysis as well as reproducibility and exchange of typing data. |
| Databáze: | OpenAIRE |
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