Endocytosis of Agarose in Mouse Peritoneal Macrophages in Vitro

Autor:	E. Johnson, R. Seljelid, J. Bøgwald, O. Larm, E. Scholander
Rok vydání:	1982
Předmět:	Male biology Macrophages Sepharose Immunology Complement System Proteins General Medicine In Vitro Techniques Endocytosis Molecular biology In vitro Mice chemistry.chemical_compound chemistry Polysaccharides biology.protein Animals Humans Ingestion Agarose Syngeneic mouse Female Antibody Incubation
Zdroj:	Scandinavian Journal of Immunology. 15:205-210
ISSN:	1365-3083 0300-9475
DOI:	10.1111/j.1365-3083.1982.tb00639.x
Popis:	We have studied the endocytosis of tritium-labelled and of non-radioactive agarose in mouse macrophages in vilro. The endocytosis was greatest and most rapid in syngeneic mouse serum and in human serum, reaching a plateau after 12 h of incubation. Ten per cent serum was the minimum concentration giving optimal ingestion. The endocytosis appeared to be regulated by mechanisms involving complement factors C3 and B. Different pretreatments of sera, inactivating or depleting C3 and B, resulted in 70–80% reduction of endocytosis, Preincubation of agarose in untreated serum increased the endocytosis of agarose in heat-inactivated serum three-fold, indicating that the essential factors were bound to agarose. Antibodies against C3 and B reduced endocytosis moderately hut significantly.
Databáze:	OpenAIRE
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