Regulation of inhibitors of differentiation family proteins by thyroid-stimulating hormone in FRTL-5 thyroid cells
| Autor: | Ju Hee Lee, Youn-Sun Bai, Yun-Jeung Kim, So Young Rha, Seul Young Kim, Young Suk Jo, Jun Hwa Hong, Y. S. Lee, Minho Shong, Heung-kyu Ro, Yun-Sun Choi, Ihn-Suk Lee, Eun Suk Hwang |
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| Rok vydání: | 2008 |
| Předmět: |
Cell type
medicine.medical_specialty endocrine system endocrine system diseases Thyroid Gland Thyrotropin Biology Thyroglobulin Proto-Oncogene Proteins c-myc chemistry.chemical_compound PAX8 Transcription Factor Phosphatidylinositol 3-Kinases Thyroid-stimulating hormone Internal medicine medicine Animals Insulin Paired Box Transcription Factors Phosphatidylinositol Receptor Promoter Regions Genetic Gene Cell Proliferation Inhibitor of Differentiation Protein 2 Kinase Id2 protein Thyroid Cell Differentiation General Medicine Rats Endocrinology medicine.anatomical_structure chemistry Gene Expression Regulation Cattle Original Article hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Journal of Korean Medical Science |
| ISSN: | 1011-8934 |
| Popis: | Members of the inhibitors of differentiation (Id) family of helix-loop-helix (HLH) proteins are known to play important roles in the proliferation and differentiation of many cell types. Thyroid-stimulating hormone (TSH) regulates proliferation and differentiation by activating TSH receptor (TSHR) in thyrocytes. In this study, we found that Id2, one of the Id family proteins, is a major target for regulation by TSH in FRTL-5 thyroid cells. TSH rapidly increases the Id2 mRNA level in FRTL-5 thyroid cells but the Id2 protein showed biphasic regulatory patterns, being transiently reduced and subsequently induced by TSH treatment. Transient reduction of Id2 protein was noted within 2 hr of TSH treatment and was mediated by proteasomal degradation. Moreover, reduced Id2 expression correlated with the activity of the phosphatidylinositol 3 kinase pathway, which is activated by TSH. Although TSH increases the activity of the Id2 promoter, TSH-induced activation of this promoter was independent of c-Myc. Id2 did not alter TTF-1- and Pax-8-mediated effects on the regulation of the Tg promoter. Thus, in summary, we found that TSH regulates Id2 expression, but that Id2 does not alter the expression of thyroid-specific genes, such as Tg, in FRTL-5 thyroid cells. |
| Databáze: | OpenAIRE |
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