Phosphorylation of proteins of synaptosome-enriched fractions of brain during short-term training experience: Biochemical characterization
| Autor: | John Eric Wilson, Edward Glassman, Willem Hendrik Gispen, Ramasamy Perumal |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Male
Radioactive Label Sucrose Nerve Tissue Proteins Phosphates Mice chemistry.chemical_compound Residue (chemistry) Avoidance Learning Animals General Environmental Science Synaptosome chemistry.chemical_classification Electroshock Histocytochemistry Nucleotides Brain Phosphoproteins Phosphate Enzyme chemistry Biochemistry Covalent bond Conditioning Operant General Earth and Planetary Sciences Phosphorylation Synaptosomes |
| Zdroj: | Behavioral Biology. 21:341-357 |
| ISSN: | 0091-6773 |
| Popis: | Incorporation of radioactive phosphate into hot HClO4 residue derived from synaptosomal particulate and soluble fractions was studied using a double-isotope technique. Increased phosphorylation was observed only in the particulate fractions of foot-shock avoidance-conditioned mice compared with quiet controls. Similar results were obtained irrespective of whether discontinuous sucrose or Ficoll—sucrose gradients were used to isolate the synaptosome-enriched fractions. Enzymatic and chemical characterization showed the radioactive label in the hot HClO4 residue to be due to phosphorus covalently bound to proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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