Pregnancy-associated changes in oligomannose oligosaccharides of human and bovine uromodulin (Tamm-Horsfall glycoprotein)
| Autor: | Charles E. Moody, Jean M. Decker, Rosalita M Smagula, Anne P. Sherblom, Andrew V. Muchmore, Herman van Halbeek |
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| Rok vydání: | 1990 |
| Předmět: |
Tamm–Horsfall protein
T cell T-Lymphocytes Molecular Sequence Data Oligosaccharides Pregnancy Proteins Biochemistry Mice Mucoproteins Pregnancy Uromodulin medicine Animals Humans Molecular Biology Chromatography High Pressure Liquid chemistry.chemical_classification biology Chemistry Cell Biology Carbohydrate moiety medicine.disease Molecular biology Glycopeptide medicine.anatomical_structure Carbohydrate Sequence Tamm-Horsfall glycoprotein biology.protein Chromatography Gel Cattle Electrophoresis Polyacrylamide Gel Female Glycoprotein Mannose Cell Division |
| Zdroj: | Glycoconjugate journal. 7(6) |
| ISSN: | 0282-0080 |
| Popis: | The urinary glycoprotein uromodulin (Tamm-Horsfall glycoprotein) exhibits a pregnancy-associated ability to inhibit antigen-specific T cell proliferation, and the activity is associated with a carbohydrate moiety [Muchmore and Decker (1985) Science 229:479-81; Hession et al., (1987) Science 237:1479-84; Muchmore, Shifrin and Decker (1987) J Immunol 138:2547-53]. We report here that the Man6(7)GlcNAc2-R glycopeptides derived from uromodulin inhibit antigen-specific T cell proliferation by 50% at 0.2-2 microM, and further studies, reported elsewhere, confirm that oligomannose glycopeptides from other sources are also inhibitory, with Man9GlcNAc2-R the most inhibitory of those tested [Muchmore et al., J Leukocyte Biol (in press)]. In this work, we have extended the observation of pregnancy-associated inhibitory activity to a second species, and have compared the oligomannose profile of Tamm-Horsfall glycoprotein (nonpregnant) with that of uromodulin (pregnant) derived from both human and bovine sources. Surprisingly, there was a pregnancy-associated decrease in the total content of oligomannose chains due predominantly to a reduction in Man5GlcNAc2-R and Man6GlcNAc2-R. Man7GlcNAc2-R, which did not decrease with pregnancy, comprised a significantly greater proportion of the total oligomannose chains in pregnant vs. nonpregnant samples from both species (human; 34.6% vs. 25.9%: bovine; 14.4% vs. 7.2%). |
| Databáze: | OpenAIRE |
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