Regulation of pel genes, major virulence factors in the plant pathogen bacterium Dickeya dadantii, is mediated by cooperative binding of the nucleoid-associated protein H-NS
| Autor: | Ouafa Zghidi-Abouzid, Elodie Hérault, Sylvie Rimsky, Sylvie Reverchon, William Nasser, Malcolm Buckle |
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| Přispěvatelé: | Chromatine et Régulation de la Pathogénie bactérienne (CRP), Microbiologie, adaptation et pathogénie (MAP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon, Laboratoire de Biologie et de Pharmacologie Appliquée (LBPA), École normale supérieure - Cachan (ENS Cachan)-Centre National de la Recherche Scientifique (CNRS), Rodrigue, Agnès |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Virulence Factors [SDV]Life Sciences [q-bio] 030106 microbiology DNA Footprinting Virulence Biology Microbiology 03 medical and health sciences Bacterial Proteins Gene expression Nucleoid Gene silencing Binding site Molecular Biology Gene ComputingMilieux_MISCELLANEOUS Polysaccharide-Lyases Binding Sites Cooperative binding Gene Expression Regulation Bacterial General Medicine Surface Plasmon Resonance biology.organism_classification Dickeya dadantii [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Cell biology DNA-Binding Proteins [SDV] Life Sciences [q-bio] 030104 developmental biology Mutation [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Gammaproteobacteria Protein Binding |
| Zdroj: | Research in Microbiology Research in Microbiology, Elsevier, 2016, 167 (4), pp.247-253 |
| ISSN: | 0923-2508 |
| Popis: | Dickeya dadantii is a pathogen infecting a wide range of plant species. Soft rot, the visible symptom, is mainly due to production of pectate lyases (Pels) that can destroy plant cell walls. Previously, we found that nucleoid-associated protein (NAP) H-NS is a key regulator of pel gene expression. The primary binding sites of this NAP have been determined here by footprinting experiments on the pelD gene, encoding an essential virulence factor. Quantitative analysis of DNAse I footprints and surface plasmon resonance imagery experiments further revealed that high-affinity binding sites initiate cooperative binding to establish the nucleoprotein structure required for gene expression silencing. Mutations in the primary binding sites resulted in reduction or loss of repression by H-NS. Overall, these data suggest that H-NS represses pelD, and by inference, other pel genes, by a cooperative binding mechanism, through oligomerization of H-NS molecules. |
| Databáze: | OpenAIRE |
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