A new genetic analysis of Ficus spp. By HAT-Random amplified Polymorphic DNA Technique

Autor: W. Phromthep
Rok vydání: 2012
Předmět:
Zdroj: Procedia Engineering. 32:1073-1079
ISSN: 1877-7058
DOI: 10.1016/j.proeng.2012.02.057
Popis: We present the genetic variation within 25 species of Ficus was result by using HAT-RAPD technique. Four single arbitrary primers, OPAS 10, OPG 13, OPLO 5 and OPWO 6, which used to amplify DNA polymorphic bands. From the total of 143 bands and 140 bands were polymorphisms. When HAT-RAPD markers were analyzed and represented in a dendro gram, which was found that Ficus was divided into two major groups and each group was divided into two subgroups. For subgroup 1.1 F. locor and F. rumphii exhibited close genetic relationship. For subgroups 2.1 and 2.2 only F. racemosa , F. racemosa var . racemosa and Ficus spp. Number 16, Ficus spp. Number 2.2 exhibited closer genetic relationship than other number.
Databáze: OpenAIRE