A novel silver (I) complex of α-keto phosphorus ylide: Synthesis, characterization, crystal structure, biomolecular interaction studies, molecular docking and in vitro cytotoxic evaluation
| Autor: | Mahzad Rahimi, Kazem Karami, Amir Abbas Momtazi-Borojeni, Mostafa Zakariazadeh, Seyed-Alireza Esmaeili, Orhan Büyükgüngör |
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| Přispěvatelé: | Ondokuz Mayıs Üniversitesi |
| Rok vydání: | 2019 |
| Předmět: |
CT-DNA binding
BSA binding Absorption spectroscopy Phosphorus ylides 010402 general chemistry 01 natural sciences Anticancer activity Fluorescence spectroscopy Analytical Chemistry Inorganic Chemistry chemistry.chemical_compound Bromide Silver trifluoromethanesulfonate Bovine serum albumin Spectroscopy chemistry.chemical_classification biology 010405 organic chemistry Chemistry Organic Chemistry 0104 chemical sciences Crystallography Förster resonance energy transfer Ylide Molecular docking biology.protein Titration |
| Zdroj: | Journal of Molecular Structure. 1177:430-443 |
| ISSN: | 0022-2860 |
| DOI: | 10.1016/j.molstruc.2018.09.063 |
| Popis: | Esmaeili, Seyed-Alireza/0000-0002-9371-4170; Momtazi-Borojeni, Amir Abaas/0000-0002-4376-1083 WOS: 000450377600049 {[Ag(FBPY)(2)](+)(FBPYH)(+)}(OTF)(2), FBPY=FPhC(O)C(H)PPh3, FBPYH =FC6H4COCH2PPh3, OTF=CF3SO3, a ylidic complex, was prepared through the reaction of silver trifluoromethanesulfonate (AgOTf) and ylide [FBPY] in 1:2 M ratio and characterized using various techniques. X-ray crystallography was used to determine the crystal structure. CT-DNA binding interaction of the synthesis compound was tested by fluorescence spectroscopy, UV-Vis absorption spectroscopy, and viscometric titration method. The data by the analysis revealed that the Ag complex could bind to DNA through the groove binding mode. The emission titration of bovine serum albumin (BSA) with the complex showed a static process for the fluorescence quenching mechanism of BSA. In addition, the donor (BSA) - acceptor (Ag complex) distance was calculated by using fluorescence resonance energy transfer (FRET). The results of competitive binding by means of Warfarin, Ibuprofen and Digoxin site markers revealed that the complex was bound to the site I of BSA. Notably, molecular docking studies were used for the determination of DNA and BSA-Ag (I) complex binding. Finally, it was shown that the complex had remarkable in vitro cytotoxicity against melanoma (B16F0) and colon carcinoma (C26) cancer cell lines, as shown by the use of MIT([3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide] colorimetric assay. (c) 2018 Elsevier B.V. All rights reserved. Isfahan University of Technology (IUT); Faculty of Arts and Sciences, Department of Physics, Ondokuz Mayrs University This research was funded by Isfahan University of Technology (IUT). Crystallography was provided by Faculty of Arts and Sciences, Department of Physics, Ondokuz Mayrs University. |
| Databáze: | OpenAIRE |
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