Cloning, purification and study of recombinant GH3 family β-glucosidase from Penicillium verruculosum
| Autor: | Ivan N. Zorov, P.V. Volkov, Alexandra M. Rozhkova, Arkady P. Sinitsyn |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cellobiose Biochemistry Substrate Specificity law.invention Fungal Proteins 03 medical and health sciences chemistry.chemical_compound Hydrolysis law Cloning Molecular Cellulose Gene chemistry.chemical_classification 030102 biochemistry & molecular biology Molecular mass biology beta-Glucosidase Aspergillus niger Penicillium General Medicine Hydrogen-Ion Concentration biology.organism_classification Recombinant Proteins Molecular Weight Kinetics 030104 developmental biology Enzyme chemistry Recombinant DNA |
| Zdroj: | Biochimie. 168:231-240 |
| ISSN: | 0300-9084 |
| DOI: | 10.1016/j.biochi.2019.11.009 |
| Popis: | A novel bgl1 gene, encoding GH3 family β-glucosidase from Penicillium verruculosum (PvBGL), was cloned and heterologously expressed in P. canescens RN3-11-7 (niaD-) strain under the control of the strong xylA gene promoter. The recombinant rPvBGL was purified and their properties were studied in comparison with those of rAnBGL from Aspergillus niger expressed previously in the same fungal host. The rPvBGL had an observed molecular mass of 90 kDa (SDS-PAGE data) and displayed the enzyme maximum activity at pH 4.6 and 65 °C. The enzyme half-life time at 60 °C was found to be 87 min. Unlike the rAnBGL, the rPvBGL was not adsorbed on microcrystalline cellulose, which gives the latter enzyme an advantage in cellulose conversion with a longer time of hydrolysis. |
| Databáze: | OpenAIRE |
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