Decreasing myocardial estrogen receptors and antioxidant activity may be responsible for increasing ischemia- and reperfusion-induced ventricular arrhythmia in older female rats
| Autor: | Salih Tunç Kaya, Canan Sapmaz, Talat Ogulcan Ozarslan, Omer Bozdogan, Azra Bozcaarmutlu, Selcuk Yasar, Didem Eksioglu |
|---|---|
| Přispěvatelé: | [Belirlenecek] |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Aging medicine.drug_class Ischemia Estrogen receptor Myocardial Reperfusion Injury 030226 pharmacology & pharmacy Antioxidants General Biochemistry Genetics and Molecular Biology Rats Sprague-Dawley Electrocardiography 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Blood serum Heart Rate Internal medicine medicine Animals Myocardial General Pharmacology Toxicology and Pharmaceutics Fulvestrant business.industry Myocardium General Medicine medicine.disease Malondialdehyde Rats 030104 developmental biology Blood pressure Endocrinology medicine.anatomical_structure Receptors Estrogen chemistry Estrogen Ventricular Fibrillation Rat Female Antioxidant business Arrhythmia medicine.drug Artery |
| Popis: | Aims: This study aimed to investigate the relationship between ischemia- and reperfusion-induced arrhythmia and blood serum estrogen levels, myocardial estrogen receptor levels, antioxidant enzyme activities, and the effects of the estrogen receptor blocker, fulvestrant (ICI 182 780). Main methods: A total of 102 female Sprague-Dawley rats of different ages (2-3, 6-7, 14-15, and 20-21 months) were used in this study. Myocardial ischemia was produced by ligation of the descending branch of the left anterior descending coronary artery, and reperfusion was produced by releasing this artery. An electrocardiogram (ECG) and blood pressure were recorded for 6 min of ischemia and 6 min of reperfusion. The levels of superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), estrogen receptor alpha (ER alpha), and estrogen receptor beta (ER beta) in myocardial tissue and 17 beta-estradiol (E2) in blood serum were measured via enzyme-linked immunosorbent assay (ELISA). The results were compared using a Mann-Whitney U test, one-way analysis of variance (ANOVA), and a student's t-test. Key findings: It is not the changes in serum estrogen levels but the decreasing myocardial estrogen receptors and antioxidant activities that could be responsible for the occurrence of more severe arrhythmia in response to reperfusion in older female rats. Significance: The death rate due to a heart attack in younger men is higher than in women. However, it equalizes after the menopausal stage in women. In this study, the reason for the increasing sudden post-menopausal death rate in women was investigated experimentally. Bolu Abant Izzet Baysal University Scientific Research Projects Coordination Unit Turkey [BAP-2016.03.01.998] This work was supported by Bolu Abant Izzet Baysal University Scientific Research Projects Coordination Unit Turkey [grant number BAP-2016.03.01.998]. WOS:000626600400027 2-s2.0-85100632588 PubMed: 33571518 |
| Databáze: | OpenAIRE |
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