Corneal angiogenesis based on different protocols of alkaline cauterization in murine models
| Autor: | Gisele Pereira Valdetaro, Karina Kamachi Kobashigawa, José Luiz Laus, Flor Diana Yokoay Claros-Chacaltana, Ivan Ricardo Martinez Padua, Marcela Aldrovani |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Time Factors Silver RD1-811 Potassium Compounds Potassium medicine.medical_treatment Cautery chemistry.chemical_element Cornea Neovascularization 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Reference Values medicine Multiple time Animals Corneal Neovascularization Rats Wistar Nitrates Neovascularization Pathologic business.industry Corneal neovascularization Corneal Angiogenesis Reproducibility of Results Rats Potassium nitrate medicine.disease eye diseases Disease Models Animal 030104 developmental biology medicine.anatomical_structure chemistry 030221 ophthalmology & optometry Cauterization Surgery sense organs medicine.symptom business |
| Zdroj: | Acta Cirurgica Brasileira, Vol 32, Iss 8, Pp 607-616 (2017) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Acta Cirúrgica Brasileira v.32 n.8 2017 Acta Cirúrgica Brasileira Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC Acta Cirurgica Brasileira, Volume: 32, Issue: 8, Pages: 607-616, Published: AUG 2017 |
| ISSN: | 0102-8650 |
| Popis: | Made available in DSpace on 2018-12-11T17:34:04Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-01-01. Added 1 bitstream(s) on 2019-10-09T18:32:57Z : No. of bitstreams: 1 S0102-86502017000800607.pdf: 830105 bytes, checksum: edeba7d554e88602c3cb31373eabd210 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Purpose: To establish and compare protocols of alkaline cauterization for inducing corneal angiogenesis in murine models. Methods: Twenty-four adult Wistar rats were distributed into four groups (G1, G2, G3, and G4). The right eye cornea from each rat was cauterized using filter paper (3 mm), soaked in a solution of silver and potassium nitrates (3:1). Cauterization times were 10 (G1 and G4), or 20 seconds (G2 and G3). Cauterized corneas were washed with Ringer’s lactate solution. The filter paper was either removed before washing (G1 and G2), or kept on the corneas (G3 and G4). Corneas were photographed at multiple time points (2, 4, 6, 8, 11, 13, and 15 days after the procedure), and neovascularization parameters were assayed. Results: Neovascularization was observed in 66% of G1 corneas, and 100% of G2, G3, and G4 corneas. On day 15, G1 corneas showed smaller vascularized areas (12.63 ± 12.59%) compared to those in the G3 (41.95 ± 17.32%) and G4 (33 ± 11.74%) (P < 0.05) groups. Conclusions: The silver and potassium nitrate solution effectively induced corneal angiogenesis. The G2, G3, and G4 protocols showed excellent reproducibility, and induced vascularization in 100% of corneas. Ophthalmology Unit Department of Veterinary Clinical Medicine and Surgery Faculty of Agrarian and Veterinary Sciences Universidade Esatdual de São Paulo FCAV-UNESP Ophthalmology Unit Department of Veterinary Clinical Medicine and Surgery Faculty of Agrarian and Veterinary Sciences Universidade Esatdual de São Paulo FCAV-UNESP FAPESP: 2009/51773-4 CNPq: 300833/2010-5 |
| Databáze: | OpenAIRE |
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