Intracellular activities of Salmonella enterica in murine dendritic cells
| Autor: | Tobias M. Lindig, Michael Hensel, Joachim Hein, Cédric Cheminay, Jonathan Jantsch, Dipshikha Chakravortty |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Salmonella typhimurium
Salmonella Genomic Islands Virulence Factors Recombinant Fusion Proteins Green Fluorescent Proteins Immunology Population Cell Culture Techniques Colony Count Microbial Virulence Endosomes medicine.disease_cause Microbiology Virulence factor Mice Bacterial Proteins Genes Reporter Virology medicine Animals Promoter Regions Genetic education Microbiology & Cell Biology education.field_of_study biology Intracellular parasite Membrane Proteins Dendritic Cells Gene Expression Regulation Bacterial biology.organism_classification Pathogenicity island Luminescent Proteins Mutagenesis Insertional Protein Transport Genes Bacterial Salmonella enterica Mutation Intracellular Molecular Chaperones |
| Zdroj: | Cellular Microbiology. 5:933-945 |
| ISSN: | 1462-5822 1462-5814 |
| DOI: | 10.1046/j.1462-5822.2003.00334.x |
| Popis: | Dendritic cells (DC) efficiently phagocytose invading bacteria, but fail to kill intracellular pathogens such as Salmonella enterica serovar Typhimurium (S. Typhimurium). We analysed the intracellular fate of Salmonella in murine bone marrow-derived DC (BM-DC). The intracellular proliferation and subcellular localization were investigated for wild-type S. Typhimurium and mutants deficient in Salmonella pathogenicity island 2 (SPI2), a complex virulence factor that is essential for systemic infections in the murine model and intracellular survival and replication in macrophages. Using a segregative plasmid to monitor intracellular cell division, we observed that, in BM-DC, S. Typhimurium represents a static, non-dividing population. In BM-DC, S. Typhimurium resides in a membrane-bound compartment that has acquired late endosomal markers. However, these bacteria respond to intracellular stimuli, because induction of SPI2 genes was observed. S. Typhimurium within DC are also able to translocate a virulence protein into their host cells. SPI2 function was not required for intracellular survival in DC, but we observed that the maturation of the Salmonella-containing vesicle is different in DC infected with wild-type bacteria and a strain deficient in SPI2. Our observations indicate that S. Typhimurium in DC are able to modify normal processes of their host cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|