LncRNA PCGEM1 Induces Ovarian Carcinoma Tumorigenesis and Progression Through RhoA Pathway
| Autor: | Lili Wang, Yang Zhao, Kai-Xuan Sun, Xue Guan, Shuo Chen, Yao Liu, Zhi-Hong Zong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
MMP2 RHOA Physiology medicine.disease_cause lcsh:Physiology lcsh:Biochemistry 03 medical and health sciences Prostate cancer 0302 clinical medicine Downregulation and upregulation Ovarian cancer Ovarian carcinoma Tumorigenesis and progression medicine Gene silencing Humans lcsh:QD415-436 RNA Neoplasm Ovarian Neoplasms biology lcsh:QP1-981 RhoA medicine.disease Neoplasm Proteins Gene Expression Regulation Neoplastic 030104 developmental biology Cell Transformation Neoplastic 030220 oncology & carcinogenesis biology.protein Cancer research Female RNA Long Noncoding Carcinogenesis rhoA GTP-Binding Protein Lncrna PCGEM1 |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 47, Iss 4, Pp 1578-1588 (2018) |
| ISSN: | 1421-9778 1015-8987 |
| Popis: | Background/Aims: Prostate cancer gene expression marker 1 (PCGEM1) is a long noncoding RNA (lncRNA) and is well known as a promoter in prostate cancer and osteoarthritis synoviocytes. However, the role PCGEM1 plays in epithelial ovarian cancer is unknown. Methods: PCGEM1 expression was examined in epithelial ovarian cancer and normal ovarian tissues using reverse transcription–PCR. Ovarian cancer cell phenotypes and genotypes were examined after PCGEM1 overexpression or downregulation in vitro; besides, the effects of PCGEM1 overexpression was also examined in vivo. Results: PCGEM1 expression level was higher in epithelial ovarian cancer tissues than in normal ovarian tissues and was positively associated with differentiation (Well vs. Mod/Poor). Upregulation of PCGEM1 induced cancer cell proliferation, migration, and invasion, but decreased cell apoptosis through upregulating RhoA, YAP (Yes-associated protein), MMP2 (matrix metalloproteinase 2), Bcl-xL, and P70S6K expression; while PCGEM1 downregulation had the opposite effect. The nude mouse xenograft assay demonstrated that PCGEM1 overexpression promoted tumor growth. Furthermore, silencing RhoA expression reversed the effect of PCGEM1 and significantly inhibited RhoA, YAP, MMP2, Bcl-xL, and P70S6K protein expression. Conclusion: In conclusion, we suggest that PCGEM1 may be an inducer in epithelial ovarian cancer tumorigenesis and progression by upregulating RhoA and the subsequent expression of YAP, P70S6K, MMP2, and Bcl-xL. |
| Databáze: | OpenAIRE |
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