An amputation resets positional information to a proximal identity in the regenerating zebrafish caudal fin
| Autor: | Leonor Saúde, António Jacinto, Ana Sofia Azevedo, Sara Sousa |
|---|---|
| Přispěvatelé: | Centro de Estudos de Doenças Crónicas (CEDOC), NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) |
| Jazyk: | angličtina |
| Předmět: |
LIMB REGENERATION
medicine.medical_treatment ved/biology.organism_classification_rank.species Gene Expression Shh Animals Genetically Modified 0302 clinical medicine RETINOIC ACID Fgf lcsh:QH301-705.5 Zebrafish 0303 health sciences biology PROLIFERATION Shh Fgf Anatomy medicine.anatomical_structure Animal Fins GROWTH Research Article Signal Transduction 03 medical and health sciences medicine Animals Regeneration Hedgehog Proteins Receptor Fibroblast Growth Factor Type 1 14. Life underwater Model organism Process (anatomy) 030304 developmental biology SONIC HEDGEHOG Caudal fin regeneration ved/biology Regeneration (biology) Fish fin Zebrafish Proteins biology.organism_classification Spinal cord Bony ray bifurcations Fibroblast Growth Factors lcsh:Biology (General) Gene Expression Regulation Amputation BLASTEMA 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | BMC Developmental Biology BMC Developmental Biology, Vol 12, Iss 1, p 24 (2012) |
| ISSN: | 1471-213X |
| DOI: | 10.1186/1471-213x-12-24 |
| Popis: | We thank Lara Carvalho and Fabio Valerio for excellent zebrafish husbandry, Andreia Pinto for histology and Antonio Temudo for imaging support. We are grateful to C. Certal and S. Pascoal for comments on the manuscript. A. S. A and S. S were supported by FCT fellowships (SFRH/BD/33179/2007 and SFER/BD/32952/2006). L. S. was supported by two FCT grants (PTDC/SAU-OBD/64628/2006 and PTDC/SAU-OBD/100202/2008) and A.J. was supported by one FCT grant (PTDC/SAU-OBD/100200/2008). Background: Zebrafish has emerged as a powerful model organism to study the process of regeneration. This teleost fish has the ability to regenerate various tissues and organs like the heart, spinal cord, retina and fins. In this study, we took advantage of the existence of an excellent morphological reference in the zebrafish caudal fin, the bony ray bifurcations, as a model to study positional information upon amputation. We investigated the existence of positional information for bifurcation formation by performing repeated amputations at different proximal-distal places along the fin. Results: We show that, while amputations performed at a long distance from the bifurcation do not change its final proximal-distal position in the regenerated fin, consecutive amputations done at 1 segment proximal to the bifurcation (near the bifurcation) induce a positional reset and progressively shift its position distally. Furthermore, we investigated the potential role of Shh and Fgf signalling pathways in the determination of the bifurcation position and observed that they do not seem to be involved in this process. Conclusions: Our results reveal that, an amputation near the bifurcation inhibits the formation of the regenerated bifurcation in the pre-amputation position, inducing a distalization of this structure. This shows that the positional memory for bony ray bifurcations depends on the proximal-distal level of the amputation. publishersversion published |
| Databáze: | OpenAIRE |
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