Survival of Campylobacter jejuni Strains from Different Origins Under Oxidative Stress Conditions: Effect of Temperature
| Autor: | Florence Rama, Martine Denis, M. Ritz, M. Federighi, F. Jugiau, Amélie Garénaux, R. de Jonge |
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| Rok vydání: | 2008 |
| Předmět: |
Paraquat
Population Colony Count Microbial medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Campylobacter jejuni Poultry Agar plate chemistry.chemical_compound Campylobacter Infections medicine Animals Humans Microaerophile education Oxygen toxicity Pathogen education.field_of_study Microbial Viability biology Temperature General Medicine biology.organism_classification medicine.disease Culture Media Oxygen Oxidative Stress chemistry Oxidative stress |
| Zdroj: | Current Microbiology. 56:293-297 |
| ISSN: | 1432-0991 0343-8651 |
| Popis: | Campylobacter jejuni is a microaerophilic pathogen but is able to survive oxidative stress conditions during its transmission to the human host. Strains of different origins (reference, poultry, or human clinical) were tested for survival under oxidative stress conditions. C. jejuni strains were grown in Mueller Hinton broth to obtain late exponential-phase cultures. Then they were exposed to 2 different stresses: (1) cultures were either plated on Columbia agar plates and exposed to atmospheric oxygen or (2) paraquat (a chemical oxidizing agent) was added to liquid cultures to reach a 500-microM concentration. Both of these experimental conditions were realized at 3 different temperatures: 4 degrees C, 25 degrees C, and 42 degrees C. Results obtained with paraquat and atmospheric oxygen were similar. Surprisingly, C. jejuni was found to be very sensitive to oxidative stress at 42 degrees C, which is its optimal growth temperature, whereas it was more resistant at 4 degrees C. A strain effect was observed, but no relationship was found between the origin of the strains and level of resistance. High temperature (42 degrees C) combined with oxidative stress allowed a rapid decrease in the C. jejuni population, whereas low temperature considerably decreased the effect of oxidative stress. |
| Databáze: | OpenAIRE |
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