Partial characterization of allergens associated with hypersensitivity to the 'green nimitti' midge (Cladotanytarsus lewisi, Diptera: Chironomidae)
| Autor: | Rosemary D. Tee, O. Cromwell, P. S. Cranston, A. B. Kay, Joan L. Longbottom |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Immunology
Chironomidae Radioallergosorbent Test otorhinolaryngologic diseases Hypersensitivity Immunology and Allergy Humans Crossed radioimmunoelectrophoresis biology Chromatofocusing Isoelectric focusing Tissue Extracts Diptera Sodium Dodecyl Sulfate Allergens Immunoglobulin E biology.organism_classification Molecular Weight Cladotanytarsus lewisi Biochemistry Sephadex Midge Electrophoresis Polyacrylamide Gel Isoelectric Focusing Rast inhibition |
| Zdroj: | Clinical allergy. 14(2) |
| ISSN: | 0009-9090 |
| Popis: | Summary Allergens in extracts of the ‘green nimitti’ midge, Cladotanytarsus lewisi Freeman (Diptera: Chironomidae), a cause of widespread hypersensitivity in the Sudan, were isolated and partially characterized by assays which depend on the binding of 125I-anti-IgE to allergen-IgE complexes. These methods included RAST inhibition, crossed radioimmunoelectrophoresis (CRIE) and rocket radioimmunoelectrophoresis (RRIE). Following Sephadex G100 chromatography the ‘major peak’ of allergcnicity as determined by RAST inhibition. RRIE and SDS-PAGE was associated with molecules of approximately 17 000 daltons. The peak eluting at Vo contained material of molecular weight 66000 daltons which also bound 125-I-anti-IgE. but had only 61% of the activity of the ‘major peak’ by RAST inhibition. By isoelectric focusing and RRIE of fractions obtained by chromatofocusing with polybuffer exchanger 94, the ‘major peak’ was associated with multiple bands with a pI range of 3.5-5.5. These results indicate that the major allergens from C. lewisi are a group of closely related acidic peptides. |
| Databáze: | OpenAIRE |
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