Effect of oregano essential oil and carvacrol on Cryptosporidium parvum infectivity in HCT-8 cells
| Autor: | Juan E. Andrade, Mark S. Kuhlenschmidt, Theresa B. Kuhlenschmidt, Shashank Gaur |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell Survival animal diseases Cryptosporidiosis Microbial Sensitivity Tests law.invention Microbiology Cell Line 03 medical and health sciences chemistry.chemical_compound law Origanum parasitic diseases Oils Volatile Humans Carvacrol Viability assay Child Incubation Essential oil Infectivity Cryptosporidium parvum Confluency biology Oocysts biology.organism_classification In vitro 030104 developmental biology Infectious Diseases chemistry Sporozoites Monoterpenes Cymenes Parasitology |
| Zdroj: | Parasitology international. 67(2) |
| ISSN: | 1873-0329 |
| Popis: | Cryptosporidium parvum is the second leading cause of persistent diarrhea among children in low-resource settings. This study examined the effect of oregano essential oil (OEO) and carvacrol (CV) on inhibition of C. parvum infectivity in vitro. HCT-8 cells were seeded (1×106) in 96-well microtiter plates until confluency. Cell viability and infectivity were assessed by seeding HCT-8 cell monolayers with C. parvum oocysts (1×104) in two modalities: 1) 4h co-culture with bioactive (0-250μg/mL) followed by washing and incubation (48h, 37°C, 5% CO2) in bioactive-free media; and 2) 4h co-culture of C. parvum oocysts followed by washing and treatment with bioactive (0-250μg/mL) during 48-h incubation. Cell viability was tested using Live/Dead™ assay whereas infectivity was measured using C. parvum-specific antibody staining via immunofluorescence detection. Loss of cell viability was observed starting at 125μg/mL and 60μg/mL for OEO and CV, respectively. Neither OEO nor CV modulated the invasion of C. parvum sporozoites in HCT-8 cells. Treatment with bioactive after invasion reduced relative C. parvum infectivity in a dose-dependent manner to 55.6±10.4% and 45.8±4.1% at 60 and 30μg/mL of OEO and CV, respectively. OEO and CV are potential bioactives to counteract C. parvum infection in children. |
| Databáze: | OpenAIRE |
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