Olfactory neuron-specific protein is translated from a large poly(A)+ mRNA
| Autor: | M Poonian, Frank L. Margolis, M Grillo, K E Rogers, W. Sydor |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Untranslated region
Immunoprecipitation Nerve Tissue Proteins chemical and pharmacologic phenomena In Vitro Techniques complex mixtures Olfactory mucosa Olfactory Mucosa Reticulocyte Olfactory Marker Protein medicine Animals Coding region RNA Messenger Protein Precursors Messenger RNA Multidisciplinary Cell-Free System biology bacterial infections and mycoses Molecular biology In vitro Rats Molecular Weight medicine.anatomical_structure Biochemistry Protein Biosynthesis biology.protein RNA bacteria Poly A Olfactory marker protein Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 82:5218-5222 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.82.15.5218 |
| Popis: | Poly(A)+ mRNA was isolated from rat olfactory mucosa and translated in a rabbit reticulocyte cell-free protein synthesizing system. Olfactory marker protein (OMP) of Mr 18,500 was faithfully produced by this system upon addition of mucosal mRNA. The protein was identified by radioimmunoprecipitation with specific anti-OMP serum and by competitive displacement of the radioactive product with authentic OMP. In addition, the immunoprecipitated product comigrated with OMP on NaDodSO4/polyacrylamide gels and on HPLC. In vitro synthesized OMP represented 0.5% of the total translational products. Total olfactory mucosal poly(A)+ mRNA is approximately 1.5-21 kilobases in size, as determined by denaturing agarose gels. Translational assays of gel-fractionated poly(A)+ mRNA demonstrated that OMP mRNA occurs in the 2.5- to 3.4-kilobase range. An mRNA of this size could code for a protein significantly larger than OMP. Since the in vitro synthesized OMP is indistinguishable in size from OMP isolated from tissue, our data indicate that OMP is synthesized directly without the intermediate formation of a larger polypeptide precursor. Thus, OMP mRNA contains untranslated regions that are four to five times larger than the coding region. |
| Databáze: | OpenAIRE |
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