Micro RNA expression profile and functional analysis reveal that mi R ‐382 is a critical novel gene of alcohol addiction
| Autor: | Shenming Wang, Jing Li, Yunhui Cheng, Yu-Wei Liu, Chunxiang Zhang, Wen Li, Xiaojun Liu, Jingyuan Li, Jiang-Hong Ye, Shanshan Qin, Eldo V. Kuzhikandathil, Chen Xiuwen, Yanzhong Guan, Ming Xiong |
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| Rok vydání: | 2013 |
| Předmět: |
DeltaFosB
dopamine receptor D1 Nucleus accumbens Biology Pharmacology Nucleus Accumbens 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Dopamine receptor D1 In vivo microRNA Animals Research Articles 030304 developmental biology Regulation of gene expression 0303 health sciences Ethanol Gene Expression Profiling Receptors Dopamine D1 MicroRNA Expression Profile microRNAs Rats Gene expression profiling Alcoholism miR-382 Gene Expression Regulation chemistry Molecular Medicine alcohol addiction Proto-Oncogene Proteins c-fos 030217 neurology & neurosurgery |
| Zdroj: | EMBO Molecular Medicine |
| ISSN: | 1757-4684 1757-4676 |
| DOI: | 10.1002/emmm.201201900 |
| Popis: | Alcohol addiction is a major social and health concern. Here, we determined the expression profile of microRNAs (miRNAs) in the nucleus accumbens (NAc) of rats treated with alcohol. The results suggest that multiple miRNAs were aberrantly expressed in rat NAc after alcohol injection. Among them, miR-382 was down-regulated in alcohol-treated rats. In both cultured neuronal cells in vitro and in the NAc in vivo, we identified that the dopamine receptor D1 (Drd1) is a direct target gene of miR-382. Via this target gene, miR-382 strongly modulated the expression of DeltaFosB. Moreover, overexpression of miR-382 significantly attenuated alcohol-induced up-regulation of DRD1 and DeltaFosB, decreased voluntary intake of and preference for alcohol and inhibited the DRD1-induced action potential responses. The results indicated that miRNAs are involved in and may represent novel therapeutic targets for alcoholism. |
| Databáze: | OpenAIRE |
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