Diversity of extracellular vesicles from different developmental stages of Fasciola hepatica
| Autor: | Dolores Bernal, Fernando Cantalapiedra, Lidia Jara, María Trelis, Christian M. Sánchez-López, Antonio Marcilla |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Future studies 030231 tropical medicine Nanoparticle tracking analysis Extracellular vesicles 03 medical and health sciences Extracellular Vesicles 0302 clinical medicine Hepatica Size exclusion chromatography parasitic diseases Fasciola hepatica Parasite hosting Animals Secretion Life Cycle Stages biology Vesicle biology.organism_classification Cell biology 030104 developmental biology Infectious Diseases Fasciola hepatica juveniles Parasitology |
| Zdroj: | INTERNATIONAL JOURNAL FOR PARASITOLOGY r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe instname |
| ISSN: | 0020-7519 |
| Popis: | The secretion of extracellular vesicles (EVs) in Fasciola hepatica adult worms was described by our group in 2012. Since then, EVs have been found in other helminths, thus providing a new paradigm for the complete understanding of host-parasite communication. However, information was lacking regarding the possible existence and role of EVs from other developmental stages of the parasite. In this study, we confirm the secretion of EVs by F. hepatica eggs and juvenile forms. EVs were isolated by size exclusion chromatography and characterised by nanoparticle tracking analysis and electron microscopy. We observed a large diversity in the morphologies of these EVs, suggesting specific functions for different subpopulations, as has been proposed in other model systems. The identification of these populations of morphologically diverse EVs will facilitate future studies aimed at biochemically characterising the different classes of these vesicles as a first step in deciphering their role in host-parasite communication. (C) 2020 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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