East Coast Fever: Titration in Cattle of Suspensions of Theileria Parva Derived from Ticks
| Autor: | M.P. Cunningham, D.E. Radley, C.G.D. Brown, A. J. Musoke, M.J. Burridge, C. Sempebwa, R.E. Purnell |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Male
Veterinary medicine Serial dilution Biopsy Theileria parva Fluorescent Antibody Technique Tick Cryopreservation Body Temperature Tissue culture Ticks parasitic diseases Animals East Coast fever Infectivity General Veterinary biology Inoculation Vaccination biology.organism_classification Antibodies Bacterial Theileriasis Immunology Cattle Female Lymph Nodes Rabbits Apicomplexa |
| Zdroj: | British Veterinary Journal. 130:336-345 |
| ISSN: | 0007-1935 |
| DOI: | 10.1016/s0007-1935(17)35836-0 |
| Popis: | SUMMARY Suspensions of Theileria parva , infective for cattle, were obtained either by grinding infected pre-fed Rhipicephalus appendiculatus in tissue culture medium and decanting the supernate, or by feeding the ticks on capillary tubes containing either tissue culture medium or foetal calf serum and pooling the material remaining in the tubes. Infective material thus obtained was either titrated immediately in cattle, or preserved in liquid nitrogen as a stabilate before titration. Dilution of either material, fresh or revived from cryopreservation, resulted in loss of infectivity at about 10 − 3 . Whilst some variable results were obtained in groups of cattle at various dilution levels with all types of material, it appeared that more even suspensions were obtained from tick feed material. In the final experiment in the series, a tick feed stabilate inoculated at dilutions of 1/50, 1/150 and 1/450 gave a graded series of responses in cattle culminating, at the highest dilution level, in protection of 7 out of 10 cattle against a lethal challenge, after minimal primary reactions. The possibilities and difficulties of using this method for protecting cattle against East Coast fever are briefly discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|