Fibrin glue mediated delivery of bone anabolic reagents to enhance healing of tendon to bone
| Autor: | Amel Dudakovic, Simon M. Cool, Endre Soreide, Lawrence J. Berglund, Sanjeev Kakar, Andre J. van Wijnen, Lars Nordsletten, Janet M. Denbeigh, Rebekah M. Samsonraj, Eric A. Lewallen |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Anabolism Bone Morphogenetic Protein 2 Connective tissue Fibrin Tissue Adhesive Biochemistry Bone morphogenetic protein 2 Article Bone and Bones Tendons 03 medical and health sciences Anabolic Agents 0302 clinical medicine medicine Animals Rats Wistar Fibrin glue Molecular Biology Fixation (histology) Bone mineral Wound Healing business.industry X-Ray Microtomography 030229 sport sciences Cell Biology Rats Surgery Tendon 030104 developmental biology medicine.anatomical_structure Ligament Tissue Adhesives business |
| Zdroj: | Journal of Cellular Biochemistry. 119:5715-5724 |
| ISSN: | 1097-4644 0730-2312 |
| Popis: | Tendon graft healing in bone tunnels for the fixation of intra-articular ligament reconstructions may limit clinical outcome by delaying healing. This study assesses the effects of hydrogel-mediated delivery of bone anabolic growth factors in a validated model of tendon-to-bone tunnel healing. Forty-five Wistar rats were randomly allocated into three groups (BMP2-treated, GSK126-treated, and placebo). All animals underwent a tendon-to-bone tunnel reconstruction. Healing was evaluated at 4 weeks by biomechanical assessment, micro-computed tomography (bone mineral density, bone volume, cross sectional area of bone tunnels), and traditional histology. Adverse events associated with the hydrogel-mediated delivery of drugs were not observed. Results of our biomechanical assessment demonstrated favorable trends in animals treated with bone anabolic factors for energy absorption (P = 0.116) and elongation (P = 0.054), while results for force to failure (P = 0.691) and stiffness (P = 0.404) did not show discernible differences. Cross sectional areas for BMP2-treated animals were reduced, but neither BMP2 nor GSK126 administration altered bone mineral density (P = 0.492) or bone volume in the bone tunnel. These results suggest a novel and positive effect of bone anabolic factors on tendon-to-bone tunnel healing. Histological evaluation confirmed absence of collagen fibers crossing the soft tissue-bone interface indicating immature graft integration as expected at this time point. Our study indicates that hydrogel-mediated delivery of BMP2 and GSK126 appears to be safe and has the potential to enhance tendon-to-bone tunnel healing in ligament reconstructions. |
| Databáze: | OpenAIRE |
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