Loss of diversity within Mycoplasma bovis isolates collected in France from bovines with respiratory diseases over the last 35 years

Autor: Claire A.M. Becker, Marie-Anne Arcangioli, François M. Thibault, Florence Tardy
Přispěvatelé: VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Laboratoire de Lyon [ANSES], Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)
Rok vydání: 2015
Předmět:
Mycoplasma bovis
Microbiology (medical)
Veterinary medicine
Genotype
Population
Cattle Diseases
Minisatellite Repeats
Multiple Loci VNTR Analysis
Biology
medicine.disease_cause
History
21st Century
Microbiology
Genetics
medicine
Animals
Mycoplasma Infections
Typing
education
Molecular Biology
Phylogeny
ComputingMilieux_MISCELLANEOUS
Ecology
Evolution
Behavior and Systematics
Genetic diversity
education.field_of_study
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
Molecular epidemiology
Genetic Variation
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
Mycoplasma
History
20th Century
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Subtyping
3. Good health
Infectious Diseases
Multilocus sequence typing
Cattle
France
Multilocus Sequence Typing
Zdroj: Infection, Genetics and Evolution
Infection, Genetics and Evolution, Elsevier, 2015, 33, pp.118-126. ⟨10.1016/j.meegid.2015.04.019⟩
ISSN: 1567-1348
1567-7257
Popis: Mycoplasma (M.) bovis has recently emerged as a major, worldwide etiological agent of bovine respiratory diseases leading to huge economic losses mainly due to high morbidity and mortality as well as poor growth rates. The spread of M. bovis infections between different animals, herds, regions or countries has been often reported to be connected to the movement of animals. However, despite recent considerable efforts, no universal subtyping method is yet available to trace M. bovis isolates circulation at an international scale. Moreover in France, the overall population diversity of M. bovis isolates has not been assessed since the early 1990s. This study was conducted to fill in these gaps. The genotypic diversity between sixty isolates collected in France over the last 35 years was assessed using two molecular subtyping methods that addressed either the long-term epidemiological relationships (Multi Locus Sequence Typing, MLST) or the genetic microvariations (Multiple Locus VNTR Analysis, MLVA) between isolates. Phenotypic diversity was also analyzed by using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) to compare the main protein patterns of isolates. All proposed subtyping approaches were optimized and led to the same pattern in the French M. bovis population that consisted of two clusters, the first one comprising isolates collected before 2000 and the second, those collected after 2000. Recent strains were further shown to be more homogeneous than older ones, which is consistent with the spread of a single clone throughout the country. Because this spread was concomitant with the emergence of multiresistant M. bovis isolates, several hypotheses are discussed to explain the homogeneity of M. bovis isolates in France, even though the M. bovis species is fully equipped to generate diversity.
Databáze: OpenAIRE
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