Improved small intestinal preservation after lazaroid U74389G treatment and cold storage in University of Wisconsin solution
| Autor: | Steven M. Katz, Vivian A. Tellis, Shuching Sun, Elaine R. Alt, R. S. Schechner, Stuart M. Greenstein |
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| Rok vydání: | 1995 |
| Předmět: |
Blood Glucose
medicine.medical_specialty Necrosis Adenosine Allopurinol Organ Preservation Solutions Urology Cold storage Lipid peroxidation chemistry.chemical_compound Raffinose medicine.artery Intestine Small medicine Animals Insulin Viaspan Cold preservation Superior mesenteric artery Intestinal Mucosa Pregnatrienes Cryopreservation Transplantation Chemistry Hemodynamics Organ Preservation Functional recovery Glutathione Small intestine Surgery Rats medicine.anatomical_structure Rats Inbred Lew medicine.symptom |
| Zdroj: | Transplantation. 59(5) |
| ISSN: | 0041-1337 |
| Popis: | The small intestine (SI) is highly sensitive to oxygen free radical-induced injury. The most common preservation solution, University of Wisconsin (UW) solution, does not adequately prevent free radical-induced injury. Lazaroids, and U74389G in particular, are a new class of compound that are potent inhibitors of superoxide-mediated lipid peroxidation. We studied the added influence of U74389G to 18-hr cold preservation of rat SI in UW solution. Three groups of rats were studied. In group 1, SI was excised and reperfused immediately. In group 2, SI was stored in UW solution at 4 degrees C for 18 hr. In group 3, U74389G was given to the SI graft before storage and again before reperfusion. Blood reperfusion of the grafts was achieved via connection to the superior mesenteric artery and portal vein of support rats. Functional recovery was assessed using a maltose tolerance test. Weight changes were calculated and histologic studies done. After 30 and 60 min of reperfusion, maltose uptake in group 3 was significantly better than that of the group 2, and returned to control levels. Significantly more tissue swelling was noted in group 3 over control, but the magnitude was less than that of group 2. Less transmural necrosis and villous blunting were noted in group 3 versus group 2; the appearance of the mucosa in group 3 approached that of group 1. We conclude that the use of U74389G treatment in addition to cold storage in UW solution improves recovery of graft function and minimizes morphologic damage to the small intestinal mucosa. |
| Databáze: | OpenAIRE |
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