Loss of Antigen Presentation in Adipose Tissue Macrophages or in Adipocytes, but Not Both, Improves Glucose Metabolism
| Autor: | Valerie P. Wright, Sarah M. Nicoloro, Joey Liu, Kajol Anandani, Michael P. Czech, Tuo Deng, Anahita Jalilvand, Alecia Blaszczak, William P. Lafuse, David Bradley, Willa A. Hsueh, Stephen M. Bergin |
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| Rok vydání: | 2019 |
| Předmět: |
CD4-Positive T-Lymphocytes
Myeloid Adipose tissue macrophages Immunology Antigen presentation Adipose tissue CD8-Positive T-Lymphocytes Article Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Adipocyte Adipocytes medicine Animals Immunology and Allergy Macrophage Mice Knockout Antigen Presentation MHC class II biology Chemistry Macrophages Histocompatibility Antigens Class II Cell biology Glucose medicine.anatomical_structure Adipose Tissue biology.protein CD8 030215 immunology |
| Zdroj: | The Journal of Immunology. 202:2451-2459 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.1801470 |
| Popis: | Macrophages, B cells, and adipocytes are among the adipose tissue (AT) APCs that differentiate and activate naive CD4+ T cells. Mice with adipocyte loss of MHC class II (MHC II) are more insulin sensitive. Because macrophages are professional APCs, mice with genetic myeloid MHC II depletion (myeloid MHC II knockout [mMHCII−/−]) were created and metabolically characterized. FITC+ glucan-coated particles (glucan-encapsulated small interfering RNA [siRNA] particles [GeRPs]) were also used to target MHC II knockout specifically in AT macrophages (ATMs). Mice with total body mMHCII−/− were generated by crossing LyzMCre with H2Ab1 floxed mice. For specific ATM depletion of H2Ab1, GeRPs containing H2Ab1 siRNA were administered to high-fat diet–fed C57BL/6 mice. Unexpectedly, mMHCII−/− mice had loss of both macrophage and adipocyte H2Ab1, one of only two Ag-presenting arms; thus, neither cell could present Ag and activate CD4+ T cells. This inability led to a reduction in AT immunosuppressive regulatory T cells, increased AT CD8+ T cells, and no improvement in systemic metabolism. Thus, with combined systemic myeloid and adipocyte MHC II loss, the impact of ATM-specific alterations in APC activity could not be delineated. Therefore, GeRPs containing H2Ab1 siRNA were administered to specifically reduce ATM H2Ab1 which, in contrast, revealed improved glucose tolerance. In conclusion, loss of either ATM or adipocyte APC function, but not both, improves systemic glucose metabolism because of maintenance of AT regulatory T cells. |
| Databáze: | OpenAIRE |
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