Incorporation of isotopic carbon into cerebral glycogen from non-glucose substrates
| Autor: | R. V. Coxon, M E Phillips |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Male
Chromatography Paper Bicarbonate Guinea Pigs chemistry.chemical_element In Vitro Techniques Biochemistry chemistry.chemical_compound Glutamates Glycogen branching enzyme Animals Carbon Radioisotopes Pyruvates Molecular Biology Incubation biology Glycogen Radiochemistry Glutamate receptor Brain Substrate (chemistry) Cell Biology Carbon In vitro Fructose-Bisphosphatase Bicarbonates Glucose chemistry biology.protein Female Research Article |
| Zdroj: | Biochemical Journal. 146:185-189 |
| ISSN: | 0264-6021 |
| DOI: | 10.1042/bj1460185 |
| Popis: | 1. Measurable incorporation of radioactive carbon from [U-14C]pyruvate, [U-14C]-glutamate and [14C]bicarbonate into the glycogen synthesized by brain slices in vitro was demonstrated. 2. The fructose diphosphatase activity of guinea-pig brain was determined and found to be about 0.03 μmol of substrate degraded/min per g of fresh tissue. 3. The specific radioactivity of the glucose carbon from glycogen relative to that of the precursor added to the incubation medium gave approximate values of 0.195 for glucose, 0.006 for pyruvate, 0.039 for glutamate and 0.001 for bicarbonate. |
| Databáze: | OpenAIRE |
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