Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages
| Autor: | Joanna Tychowski, Farshad Tamari, Laura Lorentzen |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cholera Toxin
Linolenic acid Cell Survival Linoleic acid General Chemical Engineering Biology medicine.disease_cause General Biochemistry Genetics and Molecular Biology Microbiology chemistry.chemical_compound Mice Cholera Immunity medicine Animals Humans Viability assay Immunity Mucosal chemistry.chemical_classification Mice Inbred BALB C General Immunology and Microbiology Macrophages General Neuroscience Cholera toxin Fatty Acids Fatty acid Epithelial Cells medicine.disease Intestines chemistry Vibrio cholerae Infection |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| DOI: | 10.3791/50491-v |
| Popis: | The positive role of fatty acids in the prevention and alleviation of non-human and human diseases have been and continue to be extensively documented. These roles include influences on infectious and non-infectious diseases including prevention of inflammation as well as mucosal immunity to infectious diseases. Cholera is an acute intestinal illness caused by the bacterium Vibrio cholerae. It occurs in developing nations and if left untreated, can result in death. While vaccines for cholera exist, they are not always effective and other preventative methods are needed. We set out to determine tolerable concentrations of three fatty acids (oleic, linoleic and linolenic acids) and cholera toxin using mouse BALB/C macrophages and human intestinal epithelial cells, respectively. We solubilized the above fatty acids and used cell proliferation assays to determine the concentration ranges and specific concentrations of the fatty acids that are not detrimental to human intestinal epithelial cell viability. We solubilized cholera toxin and used it in an assay to determine the concentration ranges and specific concentrations of cholera toxin that do not statistically decrease cell viability in BALB/C macrophages. We found the optimum fatty acid concentrations to be between 1-5 ng/μl, and that for cholera toxin to be < 30 ng per treatment. This data may aid future studies that aim to find a protective mucosal role for fatty acids in prevention or alleviation of cholera infections. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|