Profile of the Roche cobas® EGFR mutation test v2 for non-small cell lung cancer
Autor: | Ana Blasco, Carlos Camps, Christian Rolfo, Pasquale Pisapia, Eloisa Jantus-Lewintre, Umberto Malapelle, Silvia Calabuig-Fariñas, Rafael Sirera, Pablo Reclusa |
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Přispěvatelé: | Malapelle, U., Sirera, R., Jantus-Lewintre, E., Reclusa, P., Calabuig-Fariñas, S., Blasco, A., Pisapia, P., Rolfo, C., Camps, C., Malapelle, Umberto, Sirera, Rafael, Jantus Lewintre, Eloísa, Reclusa, Pablo, Calabuig Fariñas, Silvia, Blasco, Ana, Pisapia, Pasquale, Rolfo, Christian, Camps, Carlos |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Lung Neoplasms EGFR DNA Mutational Analysis Real-Time Polymerase Chain Reaction medicine.disease_cause Bioinformatics Genome Pathology and Forensic Medicine resistance 03 medical and health sciences 0302 clinical medicine Carcinoma Non-Small-Cell Lung Genetics Humans Medicine Epidermal growth factor receptor Liquid biopsy Lung cancer Molecular Biology cobas® Mutation liquid biopsy biology Reverse Transcriptase Polymerase Chain Reaction business.industry Molecular pathology medicine.disease TKI ErbB Receptors 030104 developmental biology 030220 oncology & carcinogenesis Cancer research biology.protein Molecular Medicine companion diagnostic Human medicine Reagent Kits Diagnostic Personalized medicine mutation business Companion diagnostic |
Zdroj: | Expert review of molecular diagnostics |
ISSN: | 1744-8352 1473-7159 |
Popis: | Introduction: The discovery of driver mutations in non-small cell lung cancer (NSCLC) has led to the development of genome-based personalized medicine. Fifteen to 20% of adenocarcinomas harbor an epidermal growth factor receptor (EGFR) activating mutation associated with responses to EGFR tyrosine kinase inhibitors (TKIs). Individual laboratories' expertise and the availability of appropriate equipment are valuable assets in predictive molecular pathology, although the choice of methods should be determined by the nature of the samples to be tested and whether the detection of only well-characterized EGFR mutations or rather, of all detectable mutations, is required.Areas covered: The EGFR mutation testing landscape is manifold and includes both screening and targeted methods, each with their own pros and cons. Here we review one of these companion tests, the Roche cobas (R) EGFR mutation test v2, from a methodological point of view, also exploring its liquid-biopsy applications.Expert commentary: The Roche cobas (R) EGFR mutation test v2, based on real time RT-PCR, is a reliable option for testing EGFR mutations in clinical practice, either using tissue-derived DNA or plasma-derived cfDNA. This application will be valuable for laboratories with whose purpose is purely diagnostic and lacking high-throughput technologies. |
Databáze: | OpenAIRE |
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