Shell-isolated nanoparticle-enhanced Raman spectroscopy for characterization of living yeast cells
| Autor: | Ieva Matulaitienė, Tatjana Charkova, Vytautas Melvydas, Gediminas Niaura, Martynas Talaikis, Ilja Ignatjev, Agnė Zdaniauskienė, Rasa Garjonytė |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Nanoparticle
Metal Nanoparticles 02 engineering and technology Metschnikowia 010402 general chemistry Photochemistry Spectrum Analysis Raman 01 natural sciences Analytical Chemistry Metal Cell wall symbols.namesake Molecule Humans Instrumentation Spectroscopy biology Chemistry 021001 nanoscience & nanotechnology biology.organism_classification Silicon Dioxide Atomic and Molecular Physics and Optics Yeast 0104 chemical sciences visual_art Molecular vibration visual_art.visual_art_medium symbols Gold 0210 nano-technology Raman spectroscopy Metschnikowia pulcherrima |
| Zdroj: | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 240 |
| ISSN: | 1873-3557 |
| Popis: | Studying the biochemistry of yeast cells has enabled scientists to understand many essential cellular processes in human cells. Further development of biotechnological and medical progress requires revealing surface chemistry in living cells by using a non-destructive and molecular structure sensitive technique. In this study shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS) was applied for probing the molecular structure of Metschnikowia pulcherrima yeast cells. Important function of studied cells is the ability to eliminate iron from growth media by precipitating the insoluble pigment pulcherrimin. Comparative SERS and SHINERS analysis of the yeast cells in combination with bare Au and shell-isolated Au@SiO2 nanoparticles were performed. It was observed that additional bands, such as adenine ring-related vibrational modes appear due to interaction with bare Au nanoparticles; the registered spectra do not coincide with the spectra where Au@SiO2 nanoparticles were used. SHINERS spectra of M. pulcherrima were significantly enhanced comparing to the Raman spectra. Based on first-principles calculations and 830-nm excited Raman analysis of pulcherrimin, the SHINERS signatures of iron pigment in yeast cells were revealed. Being protected from direct interaction of metal with adsorbate, Au@SiO2 nanoparticles yield reproducible and reliable vibrational signatures of yeast cell wall constituents. |
| Databáze: | OpenAIRE |
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